Effect of antioxidant procyanidin b2 (pcb2) on ovine oocyte developmental potential in response to in vitro maturation (ivm) and vitrification stress.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-03-01

J Bai, J Li, L Wang, S Hao, Y Guo, Y Liu, Z Zhang, H Li, W Q Sun, G Shi, P Wan, X Fu

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Abstract

Background: It was demonstrated that external stress, such as in vitro maturation (IVM) and vitrification process can induce significantly reduced development capacity in oocytes. Previous studies indicated that antioxidants play a pivotal part in the acquisition of adaptation in changed conditions. At present, the role of the natural potent antioxidant PCB2 in response to IVM and vitrification during ovine oocyte manipulation has not been explored.

Objective: To investigate whether PCB2 treatment could improve the developmental potential of ovine oocytes under IVM and vitrification stimuli.

Materials and methods: The experiment was divided into two parts. Firstly, the effect of PCB2 on the development of oocytes during IVM was evaluated. Un-supplemented and 5 ug per mL PCB2-supplemented in the IVM solution were considered as control and experimental groups (C + 5 ug per mL PCB2). The polar body extrusion (PBE) rate, mitochondrial membrane potential (MMP), ATP, reactive oxygen species (ROS) levels and early apoptosis of oocytes were measured after IVM. Secondly, we further determine whether PCB2 could improve oocyte quality under vitrification stress. The survival rate, PBE rate and early apoptosis of oocytes were compared between fresh group, vitrified group and 5 ug per mL PCB2-supplemented in the IVM solution after vitrification (V + 5 ug per mL PCB2).

Results: Compared to the control group, adding PCB2 significantly increased PBE rate (79.4% vs. 62.8%, P < 0.01) and MMP level (1.9 +/- 0.08 vs. 1.3 +/- 0.04, P < 0.01), and decreased ROS level (47.1 +/- 6.3 vs. 145.3 +/- 8.9, P < 0.01). However, there was no significant difference in ATP content and early apoptosis. Compared to the fresh group, vitrification significantly reduced oocytes viability (43.0% vs. 90.8%, P < 0.01) as well as PBE rate (24.2% vs. 60.6%, P < 0.05). However, 5 ug per mL PCB2-supplemention during maturation had no effect on survival, PBE or early apoptosis in vitrified oocytes.

Conclusion: PCB2 could effectively antagonise the oxidative stress during IVM and promote oocyte development. DOI: 10.54680/fr23210110412.

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抗氧化剂原花青素b2（pcb2）对体外成熟（ivm）和玻璃化应激下绵羊卵母细胞发育潜力的影响。

背景：体外成熟（IVM）和玻璃化过程等外部应激可导致卵母细胞发育能力显著降低。先前的研究表明，抗氧化剂在获得适应变化条件的过程中发挥着关键作用。目前，天然强效抗氧化剂PCB2在绵羊卵母细胞操作过程中对IVM和玻璃化反应的作用尚未得到探索。目的：探讨PCB2处理是否能提高体外受精和玻璃化刺激下绵羊卵母细胞的发育潜力。材料与方法：实验分为两部分。首先，评价了PCB2对体外受精卵母细胞发育的影响。未补充和IVM溶液中补充5 ug/mL PCB2被视为对照组和实验组（C+5 ug/mL PCB2）。测定IVM后卵母细胞的极体挤出率（PBE）、线粒体膜电位（MMP）、ATP、活性氧（ROS）水平和早期凋亡。其次，我们进一步确定PCB2是否能在玻璃化应激下提高卵母细胞质量。结果：与对照组相比，添加PCB2显著提高了卵母细胞的PBE率（79.4%对62.8%，P<0.01）和MMP水平（1.9+/-0.08对1.3+/-0.04，P<0.01），ROS水平下降（47.1+/-6.3 vs.145.3+/-8.9，P<0.01），但ATP含量和早期细胞凋亡无显著差异。与新鲜组相比，玻璃化处理显著降低了卵母细胞的活力（43.0%对90.8%，P<0.01）和PBE率（24.2%对60.6%，P<0.05）。然而，在成熟过程中每毫升补充5微克PCB2对玻璃化处理的卵母细胞存活、PBE或早期凋亡没有影响。结论：PCB2能有效拮抗IVM过程中的氧化应激，促进卵母细胞发育。DOI:10.54680/fr23210110412。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.