Interactions among sucrose and concentrations of serum fetal bovine on the cryopreservation of somatic cells derived from red-rumped agoutis.

IF 1 4区 生物学 Q3 BIOLOGY Cryo letters Pub Date : 2023-03-01
E A Praxedes, M B Silva, L R Medeiros de Oliveira, J V da Silva Viana, A F Pereira
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Abstract

Background: The synergistic action among the different extracellular cryoprotectants could improve somatic cell quality after thawing and provide bases for the formation of biobanks for red-rumped agoutis.

Objective: This study evaluated the interactions among sucrose (SUC) and concentrations of serum fetal bovine (FBS) on the cryopreservation of somatic cells derived from red-rumped agoutis.

Materials and methods: Cells were cryopreserved with 10% dimethyl sulfoxide and different concentrations of FBS (10%, 40%, and 90%) with or without 0.2 M SUC, totaling six comparison groups. Non-cryopreserved cells were used as a control. Cells were evaluated for viability, metabolic activity, proliferative activity, reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis levels.

Results: No difference was observed among cryopreserved with DMSO containing (10FBS, 10FBS-SUC, 40FBS, 40FBS-SUC, 90FBS, 90FBS-SUC) and non-cryopreserved groups for viability, metabolic activity, proliferative activity, and ROS levels. Interestingly, only cells cryopreserved with 90% FBS and SUC maintained the mitochondrial membrane potential like the control. This indicates that at high concentrations of FBS, SUC contributes to the maintenance of this parameter in cryopreserved cells. Moreover, at concentrations of 10% and 40% of FBS, SUC contributed to the maintenance of viability evaluated by the levels of apoptosis evaluated after thawing. In summary, we verified that 90% FBS and 0.2 M SUC promote greater ability of cells after thawing. Additionally, SUC positively acts in cryopreservation solutions containing 10% and 40% FBS.

Conclusion: This information is essential to an understanding of the mechanisms involved in the interactions of extracellular cryoprotectants in somatic cell cryopreservation solutions of red-rumped agoutis. DOI: 10.54680/fr23210110212.

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蔗糖和血清胎牛浓度对红臀阿古斯体细胞冷冻保存的影响。
背景:不同细胞外冷冻保护剂之间的协同作用可以改善解冻后的体细胞质量,并为红臀刺鼠生物库的形成提供基础。目的:研究蔗糖(SUC)和血清胎牛(FBS)浓度对红臀阿戈特体细胞冷冻保存的影响。材料和方法:用10%二甲基亚砜和不同浓度的FBS(10%、40%和90%)冷冻保存细胞,加入或不加入0.2M SUC,共6个对照组。使用未冷冻保存的细胞作为对照。评估细胞的活力、代谢活性、增殖活性、活性氧(ROS)、线粒体膜电位和细胞凋亡水平。结果:用含有DMSO的DMSO(10FBS、10FBS-SUC、40FBS、40FBS-SUC、90FBS、90FBS-SUCs)冷冻保存组和非冷冻保存组在活力、代谢活性、增殖活性和ROS水平方面没有差异。有趣的是,只有用90%FBS和SUC冷冻保存的细胞才能像对照一样保持线粒体膜电位。这表明,在高浓度的FBS下,SUC有助于维持冷冻保存细胞中的这一参数。此外,在10%和40%的FBS浓度下,SUC有助于通过解冻后评估的细胞凋亡水平来评估生存能力的维持。总之,我们验证了90%的FBS和0.2 M的SUC在解冻后促进细胞更大的能力。此外,SUC在含有10%和40%FBS的冷冻保存溶液中具有积极作用。DOI:10.54680/fr23210110212。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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