Molecular investigation of ready-to-eat salads for Giardia duodenalis and Cryptosporidium spp. in Portugal

IF 2.9 Q2 PARASITOLOGY Food and Waterborne Parasitology Pub Date : 2023-03-01 DOI:10.1016/j.fawpar.2023.e00190

Clarissa Perez Faria , Adelaide Pereira , Daniela Almeida , Miguel Pinto , Ágata Lourenço , Maria do Céu Sousa

{"title":"Molecular investigation of ready-to-eat salads for Giardia duodenalis and Cryptosporidium spp. in Portugal","authors":"Clarissa Perez Faria , Adelaide Pereira , Daniela Almeida , Miguel Pinto , Ágata Lourenço , Maria do Céu Sousa","doi":"10.1016/j.fawpar.2023.e00190","DOIUrl":null,"url":null,"abstract":"<div>Foodborne outbreaks are often associated with the consumption of salads. However, published studies on the detection of foodborne pathogens in ready-to-eat salads are scarce. The aim of this study was to detect Giardia duodenalis and Cryptosporidium DNA in ready-to-eat salads, by applying techniques of molecular biology to study the frequency of contamination in salads. A total of 100 packages of ready-to-eat salads containing assorted leafy green vegetables were randomly purchased from hypermarkets located in central regions of Portugal (Coimbra and Viseu). Nested-PCR and qPCR methods were used to detect G. duodenalis and Cryptosporidium DNA. Species and assemblages of the parasites were identified by sequence analysis and PCR. Eighteen of the 100 samples (18%) were positive for G. duodenalis and twelve were sequenced and identified as assemblage A. Cryptosporidium spp. were not detected in any salads. Overall, pre-harvest and post-harvest preventive measures may be need for G. duodenalis control throughout the food production industry, from the field to consumers.</div>","PeriodicalId":37941,"journal":{"name":"Food and Waterborne Parasitology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Waterborne Parasitology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405676623000033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Foodborne outbreaks are often associated with the consumption of salads. However, published studies on the detection of foodborne pathogens in ready-to-eat salads are scarce. The aim of this study was to detect Giardia duodenalis and Cryptosporidium DNA in ready-to-eat salads, by applying techniques of molecular biology to study the frequency of contamination in salads. A total of 100 packages of ready-to-eat salads containing assorted leafy green vegetables were randomly purchased from hypermarkets located in central regions of Portugal (Coimbra and Viseu). Nested-PCR and qPCR methods were used to detect G. duodenalis and Cryptosporidium DNA. Species and assemblages of the parasites were identified by sequence analysis and PCR. Eighteen of the 100 samples (18%) were positive for G. duodenalis and twelve were sequenced and identified as assemblage A. Cryptosporidium spp. were not detected in any salads. Overall, pre-harvest and post-harvest preventive measures may be need for G. duodenalis control throughout the food production industry, from the field to consumers.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

葡萄牙十二指肠贾第鞭毛虫和隐孢子虫即食沙拉的分子研究

食源性疫情通常与食用沙拉有关。然而，已发表的关于在即食沙拉中检测食源性病原体的研究很少。本研究的目的是通过应用分子生物学技术研究沙拉中的污染频率，检测即食沙拉中的十二指肠贾第鞭毛虫和隐孢子虫DNA。从葡萄牙中部地区（Coimbra和Viseu）的大卖场随机购买了100包含有各种绿叶蔬菜的即食沙拉。采用巢式聚合酶链式反应和qPCR方法检测十二指肠和隐孢子虫的DNA。通过序列分析和聚合酶链式反应鉴定了寄生虫的种类和组合。100个样本中有18个（18%）对十二指肠G.denalis呈阳性，12个被测序并鉴定为A.隐孢子虫。在任何沙拉中都没有检测到。总的来说，从田间到消费者，整个食品生产行业可能需要在收获前和收获后采取预防措施来控制十二指肠溃疡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.