Toxoplasma and Toxocara seropositivity in juvenile idiopathic arthritis and its relation to disease activity and type of therapies

IF 2.9 Q2 PARASITOLOGY Food and Waterborne Parasitology Pub Date : 2023-06-01 DOI:10.1016/j.fawpar.2023.e00195

Doaa A. Salem , Ahmed Hassan Al-Ghamdi , Jameel Mohamed Alghamdi , Amira Ismail , Bakheet A. Alghamdi , Eman Abdelrazek

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引用次数: 1

Abstract

Juvenile idiopathic arthritis (JIA) is the most frequently encountered autoimmune rheumatic disease in children. To our knowledge, this is the first study aimed to estimate the frequency of Toxoplasma gondii (T. gondii) and Toxocara seropositivity in JIA and assess its relation to the disease activity, IL-10 levels, and type of the received therapies. This study was conducted on 43 JIA patients and 50 cases as a control group. All participants were evaluated by disease activity score (JADAS-27), and the presence of specific IgG and IgM antibodies against T. gondii and IgG against Toxocara species using an enzyme-linked immunosorbent assay. IL-10 serum levels were measured using an ELISA kit. The results show that JIA patients have significantly higher seropositivity for anti-T. gondii IgG compared to control subjects (p = 0.02) and a non-significant difference for Toxocara seropositivity (p = 0.41). All participants were negative for IgM anti-Toxoplasma gondii. Demographic parameters did not significantly affect these seroprevalence frequencies (p > 0.05). IL-10 was significantly higher among JIA patients compared to controls (p = 0.007) and seropositive anti-T. gondii JIA exhibited significantly higher IL-10 levels compared to seronegative ones (p = 0.03). Seropositive anti-T. gondii IgG JIA patients had a significantly higher disease activity score (JADAS-27) than seronegative anti-T. gondii IgG cases (p = 0.02). There was a significant positive correlation between anti-T. gondii IgG and JADAS-27 score (p = 0.009). A significant association was detected between T. gondii infection and DMARDs including the biological therapies (p < 0.05). Overall, this study supports a possible association between T. gondii infection and JIA, IL-10, disease activity score, and DMARDs therapies. It is possible that IL-10 plays a role in the development of JIA and contributes to persistent asymptomatic infection with T. gondii in JIA patients. As a result, a recommendation for screening tests for T. gondii infection among JIA patients is crucial before and during commencing DMARDs therapies and closely monitoring early signs of infection.

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幼年特发性关节炎弓形虫和弓形虫血清阳性及其与疾病活动性和治疗类型的关系

幼年特发性关节炎（JIA）是儿童最常见的自身免疫性风湿性疾病。据我们所知，这是第一项旨在估计JIA中弓形虫（T.gondii）和弓形虫血清阳性频率的研究，并评估其与疾病活性、IL-10水平和接受治疗类型的关系。本研究以43例JIA患者和50例作为对照组。所有参与者都通过疾病活性评分（JADAS-27）、针对弓形虫的特异性IgG和IgM抗体的存在以及使用酶联免疫吸附测定法针对弓形虫物种的IgG进行评估。使用ELISA试剂盒测量IL-10血清水平。结果表明，JIA患者血清抗T抗体阳性率明显增高。弓形虫IgG与对照组相比（p=0.02），弓形虫血清阳性率无显著差异（p=0.041）。所有参与者的IgM抗弓形虫抗体均为阴性。人口统计学参数对这些血清流行率没有显著影响（p＞0.05）。与对照组（p＝0.007）和血清阳性抗-T相比，JIA患者的IL-10显著更高。弓形虫JIA的IL-10水平明显高于血清阴性者（p=0.03）。弓形虫IgG JIA患者的疾病活动性评分（JADAS-27）显著高于血清阴性的抗T。弓形虫IgG病例（p=0.02）。弓形虫IgG和JADAS-27评分（p=0.009）。检测到弓形虫感染与DMARDs（包括生物疗法）之间存在显著关联（p<；0.05）。总体而言，本研究支持弓形虫感染和JIA、IL-10、疾病活动评分和DMARDs疗法之间可能存在关联。IL-10可能在JIA的发展中发挥作用，并导致JIA患者持续无症状感染弓形虫。因此，在开始DMARD治疗之前和期间，建议在JIA患者中进行弓形虫感染筛查测试，并密切监测感染的早期迹象，这一点至关重要。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.