{"title":"Fluorescence Spectroscopy","authors":"Claudia Y. Lee","doi":"10.1002/9780470089941.et0204s02","DOIUrl":null,"url":null,"abstract":"<p>Fluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit focuses on methods that can be applied to biological studies. Starting with the preparation of fluorescently conjugated antibodies, the first part explains the physical principles of fluorescence and the features in an absorption spectrum, and how these are used to measure the degree of labeling on conjugated antibodies. The protocols describe how to obtain and interpret a fluorescence emission spectrum and utilize this information as a fluorescence indicator for protein detection. <i>Curr. Protoc. Essential Lab. Tech</i>. 2:2.4.1-2.4.29. © 2009 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et0204s02","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et0204s02","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Fluorescence is an extremely powerful tool in modern biology, physics, and chemistry laboratories. This unit focuses on methods that can be applied to biological studies. Starting with the preparation of fluorescently conjugated antibodies, the first part explains the physical principles of fluorescence and the features in an absorption spectrum, and how these are used to measure the degree of labeling on conjugated antibodies. The protocols describe how to obtain and interpret a fluorescence emission spectrum and utilize this information as a fluorescence indicator for protein detection. Curr. Protoc. Essential Lab. Tech. 2:2.4.1-2.4.29. © 2009 by John Wiley & Sons, Inc.
荧光光谱
荧光在现代生物学、物理学和化学实验室中是一种非常强大的工具。本单元着重于可以应用于生物学研究的方法。从荧光偶联抗体的制备开始，第一部分解释了荧光的物理原理和吸收光谱的特征，以及如何使用这些来测量偶联抗体的标记程度。该协议描述了如何获得和解释荧光发射光谱，并利用该信息作为蛋白质检测的荧光指标。咕咕叫。Protoc。基本的实验室。科技,2:2.4.1-2.4.29。©2009 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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