{"title":"Centrifugation","authors":"Sean R. Gallagher","doi":"10.1002/9780470089941.et0501s9","DOIUrl":null,"url":null,"abstract":"<p>Centrifugation is a ubiquitous technique in the life science laboratory that uses specialized equipment (centrifuges) to spin, or centrifuge, samples in rotors, generating centrifugal force for a range of applications that include pelleting of precipitates, isolating organelles, and protein and DNA purification. The unit describes basic applications, definitions, and references, and provides up-to-date tables for rotor specifications from major suppliers. © 2014 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et0501s9","citationCount":"41","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et0501s9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 41
Abstract
Centrifugation is a ubiquitous technique in the life science laboratory that uses specialized equipment (centrifuges) to spin, or centrifuge, samples in rotors, generating centrifugal force for a range of applications that include pelleting of precipitates, isolating organelles, and protein and DNA purification. The unit describes basic applications, definitions, and references, and provides up-to-date tables for rotor specifications from major suppliers. © 2014 by John Wiley & Sons, Inc.
离心分离
离心是生命科学实验室中普遍使用的一种技术,它使用专门的设备(离心机)在转子中旋转或离心样品,产生离心力,用于一系列应用,包括沉淀物的颗粒化,分离细胞器,蛋白质和DNA纯化。该单元描述了基本的应用、定义和参考,并提供了主要供应商转子规格的最新表。©2014 by John Wiley &儿子,Inc。
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