{"title":"Overview of Digital Electrophoresis Analysis","authors":"Butch Moomaw, Scott Medberry, Sean R. Gallagher","doi":"10.1002/9780470089941.et0705s9","DOIUrl":null,"url":null,"abstract":"<p>Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of CCD technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis. © 2014 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et0705s9","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et0705s9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of CCD technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis. © 2014 by John Wiley & Sons, Inc.
数字电泳分析概述
数字成像是记录和分析蛋白质和DNA电泳分离的首选方法。使用基于ccd的相机可以快速获得凝胶电泳的数字图像，并且可以方便地使用图像分析软件对图像进行存档和分析。本概述定义了成像的重要关键术语和计算，解释了捕获过程，回顾了用于图像捕获的CCD技术的范围，并介绍了用于一维和二维数字图像分析的软件和方法。©2014 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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