{"title":"Beyond Simple Homology Searches: Multiple Sequence Alignments and Phylogenetic Trees","authors":"Rebecca A. Zufall","doi":"10.1002/9780470089941.et1103s01","DOIUrl":null,"url":null,"abstract":"<p>Phylogenetic trees represent hypotheses about evolutionary relationships between organisms or nucleotide or amino acid sequences. Because the best BLAST hit often does not represent the most closely related sequence, phylogenetic analyses are an essential extension of inquiry into any new protein or gene. In this unit, the reader will first learn how to create a multiple sequence alignment using ClustalX. He or she will then learn how to use that alignment to build a neighbor-joining phylogeny using the program Geneious. Finally, the user will learn how to interpret the phylogeny in light of the research questions. <i>Curr. Protoc. Essential Lab. Tech</i>. 1:11.3.1-11.3.17. © 2009 by John Wiley & Sons, Inc.</p>","PeriodicalId":500994,"journal":{"name":"Current Protocols Essential Laboratory Techniques","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470089941.et1103s01","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols Essential Laboratory Techniques","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780470089941.et1103s01","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
Phylogenetic trees represent hypotheses about evolutionary relationships between organisms or nucleotide or amino acid sequences. Because the best BLAST hit often does not represent the most closely related sequence, phylogenetic analyses are an essential extension of inquiry into any new protein or gene. In this unit, the reader will first learn how to create a multiple sequence alignment using ClustalX. He or she will then learn how to use that alignment to build a neighbor-joining phylogeny using the program Geneious. Finally, the user will learn how to interpret the phylogeny in light of the research questions. Curr. Protoc. Essential Lab. Tech. 1:11.3.1-11.3.17. © 2009 by John Wiley & Sons, Inc.
超越简单的同源性搜索:多序列比对和系统发育树
系统发育树代表生物体之间或核苷酸或氨基酸序列之间的进化关系的假设。因为最好的BLAST序列通常并不代表最密切相关的序列，系统发育分析是研究任何新蛋白质或基因的必要延伸。在本单元中，读者将首先学习如何使用ClustalX创建多序列对齐。然后，他或她将学习如何使用该对齐来使用genius程序构建邻居连接的系统发育。最后，用户将学习如何根据研究问题来解释系统发育。咕咕叫。Protoc。基本的实验室。科技,1:11.3.1-11.3.17。©2009 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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