Using Comparative Proteomics to Identify Protein Signatures in Clear Cell Renal Cell Carcinoma.

IF 2.6 4区医学 Q2 GENETICS & HEREDITY Cancer Genomics & Proteomics Pub Date : 2023-11-01 DOI:10.21873/cgp.20408

Juhee Park, Eun Hye Lee, Hyunchae Sim, Ann-Yae Na, So Young Choi, Jae-Wook Chung, Yun-Sok Ha, Tae Gyun Kwon, Sangkyu Lee, Jun Nyung Lee

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Abstract

Background/aim: Renal cell carcinoma (RCC) is one of the most commonly diagnosed cancers in the world. Approximately 25-30% of patients identified with initial kidney cancer will have metastasized tumors, thus 5-year survival rates for these patients are poor. Therefore, biomarker research is required to identify and predict molecular signatures in RCC.

Materials and methods: To address this, we used a mass spectrometry (MS)-based proteomics approach to identify proteins related to clear cell RCC (ccRCC) tissues from patients with T1G2, T1G3, T3G2, T3G3, and metastatic RCC (mRCC) stages.

Results: We identified and quantified 2,608 and 2,463 proteins, respectively, in ccRCC tissue and identified 1,449 differentially expressed proteins (DEPs). Bioinformatics analysis revealed that serpin family A member 3 (SERPINA3) qualified as biomarker for ccRCC progression. Using indirect enzyme-linked immunosorbent assay (ELISA), immunoblotting, and immunohistochemistry assays it was found that SERPINA3 expression levels in ccRCC tissues were much higher in stages before metastasis.

Conclusion: Comparative proteomics analysis of ccRCC tissues provided new evidence of SERPINA3 association with ccRCC progression.

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使用比较蛋白质组学鉴定透明细胞肾细胞癌中的蛋白质特征。

背景/目的：肾细胞癌（RCC）是世界上最常见的癌症之一。大约25-30%的癌症早期患者会有转移性肿瘤，因此这些患者的5年生存率很低。因此，需要进行生物标志物研究来识别和预测RCC的分子特征。材料和方法：为了解决这一问题，我们使用基于质谱（MS）的蛋白质组学方法来鉴定T1G2、T1G3、T3G2、T3G3和转移性RCC（mRCC）分期患者的透明细胞RCC（ccRCC）组织相关的蛋白质。结果：我们在ccRCC组织中分别鉴定和定量了2608和2463种蛋白质，并鉴定了1449种差异表达蛋白质（DEP）。生物信息学分析显示，serpin家族A成员3（SERPINA3）符合ccRCC进展的生物标志物。使用间接酶联免疫吸附试验（ELISA）、免疫印迹和免疫组织化学试验发现，在转移前的阶段，ccRCC组织中SERPINA3的表达水平要高得多。结论：ccRCC组织的比较蛋白质组学分析为SERPINA3与ccRCC进展的关系提供了新的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Genomics & Proteomics ONCOLOGY-GENETICS & HEREDITY

CiteScore

5.00

自引率

8.00%

发文量

期刊介绍： Cancer Genomics & Proteomics (CGP) is an international peer-reviewed journal designed to publish rapidly high quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research. In this site you may find information concerning the editorial board, editorial policy, issue contents, subscriptions, submission of manuscripts and advertising. The first issue of CGP circulated in January 2004. Cancer Genomics & Proteomics is a journal of the International Institute of Anticancer Research. From January 2013 CGP is converted to an online-only open access journal. Cancer Genomics & Proteomics supports (a) the aims and the research projects of the INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH and (b) the organization of the INTERNATIONAL CONFERENCES OF ANTICANCER RESEARCH.