The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of Cryptosporidium Spp.

IF 0.9 4区医学 Q4 PARASITOLOGY Iranian Journal of Parasitology Pub Date : 2023-07-01 DOI:10.18502/ijpa.v18i3.13754

Mehran Mirzaghavami, Javid Sadraei, Majid Pirestani, Saeed Bahadory

{"title":"The Role of Some Free-Ranging Animals in the Transmission of Multi-Host Species of Cryptosporidium Spp.","authors":"Mehran Mirzaghavami, Javid Sadraei, Majid Pirestani, Saeed Bahadory","doi":"10.18502/ijpa.v18i3.13754","DOIUrl":null,"url":null,"abstract":"Background: We aimed to characterize Cryptosporidium spp. in rats, cats, pigeons, and crows.Methods: Fifty-five animal origin Cryptosporidium spp. genome were identified, genotyped and confirmed by nested PCR and of RFLP-PCR analysis as well as sequenced based on 18s rRNA and gp60 genes in Tehran (2012-2019). Finally, the phylogenetic analysis was performed by MEGA software (version 7).Results: By the molecular method, Cryptosporidium spp. were detected in 24 (15.2%), 15 (15%), 2 (2%) and 13 (13%) cases of wild rats, cat, pigeon, and crow, respectively. Among the identified species by the RFLP pattern, most isolates were identified as C. parvum (24/157) 17.8% in rats, (15/100) 15% in cats, (13/100) 13%in crew and (2/100) 2% in pigeons; and the rest of the cases were C. muris and C. felis. The results of sequencing did not prove the existence of C. parvum, C. felis, C. muris, and rat genotype. Subtyping of C. parvum was indicated that the dominant subtype family belongs to the IId family and the subtype A20G1 was the most common subtype detected in all hosts while A19G1 was detected in one isolate of cat and pigeon.Conclusion: Free-ranging animals are infected by species/subtype of Cryptosporidium, which can infect humans. This shows by itself the hygienic importance of the free-ranging animals in urban ecosystems. In the transmission of human cryptosporidiosis, the multi-host Cryptosporidium species such as C. parvum, C. felis, and C. muris can be transferred potentially from these animals to humans.","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"18 3","pages":"313-323"},"PeriodicalIF":0.9000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6b/c9/IJPA-18-313.PMC10597881.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v18i3.13754","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: We aimed to characterize Cryptosporidium spp. in rats, cats, pigeons, and crows.

Methods: Fifty-five animal origin Cryptosporidium spp. genome were identified, genotyped and confirmed by nested PCR and of RFLP-PCR analysis as well as sequenced based on 18s rRNA and gp60 genes in Tehran (2012-2019). Finally, the phylogenetic analysis was performed by MEGA software (version 7).

Results: By the molecular method, Cryptosporidium spp. were detected in 24 (15.2%), 15 (15%), 2 (2%) and 13 (13%) cases of wild rats, cat, pigeon, and crow, respectively. Among the identified species by the RFLP pattern, most isolates were identified as C. parvum (24/157) 17.8% in rats, (15/100) 15% in cats, (13/100) 13%in crew and (2/100) 2% in pigeons; and the rest of the cases were C. muris and C. felis. The results of sequencing did not prove the existence of C. parvum, C. felis, C. muris, and rat genotype. Subtyping of C. parvum was indicated that the dominant subtype family belongs to the IId family and the subtype A20G1 was the most common subtype detected in all hosts while A19G1 was detected in one isolate of cat and pigeon.

Conclusion: Free-ranging animals are infected by species/subtype of Cryptosporidium, which can infect humans. This shows by itself the hygienic importance of the free-ranging animals in urban ecosystems. In the transmission of human cryptosporidiosis, the multi-host Cryptosporidium species such as C. parvum, C. felis, and C. muris can be transferred potentially from these animals to humans.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

几种自由放养动物在隐孢子虫多宿主传播中的作用。

背景：我们旨在鉴定大鼠、猫、鸽子和乌鸦体内的隐孢子虫属。方法：在德黑兰（2012-2019年），通过套式PCR和RFLP-PCR分析对55种动物来源的隐孢子虫进行了基因组鉴定、基因分型和确认，并基于18s rRNA和gp60基因进行了测序。结果：采用分子生物学方法，在野生大鼠、猫、鸽和乌鸦中分别检出隐孢子虫24例（15.2%）、15例（15%）、2例（2%）和13例（13%）。在RFLP模式鉴定的物种中，大多数分离株在大鼠中鉴定为细小C.parvum（24/157）17.8%，在猫中鉴定为（15/100）15%，在船员中鉴定为13%，在鸽子中鉴定为2%；其余病例为C.muris和C.felis。测序结果并没有证明细小C.parvum、猫C.felis、鼠C.muris和大鼠基因型的存在。细小梭菌的亚型表明，优势亚型家族属于IId家族，A20G1亚型是在所有宿主中检测到的最常见的亚型，而A19G1在猫和鸽子的一个分离株中检测到。结论：自由放养动物感染隐孢子虫的种/亚型，可感染人类。这本身就表明了自由放养动物在城市生态系统中的卫生重要性。在人类隐孢子虫病的传播中，多宿主隐孢子虫物种，如细小隐孢子虫、猫隐孢子虫和鼠隐孢子虫，可能会从这些动物转移到人类。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.