Darshana Kottahachchi , Lallindra Gooneratne , Anil Jayasekera , Dorota Muth-Pawlak , Robert Moulder , Susumu Y. Imanishi , Ari Ariyaratne , Anne Rokka , Garry L. Corthals
{"title":"Quantitative analysis of the erythrocyte membrane proteins in polycythemia vera patients treated with hydroxycarbamide","authors":"Darshana Kottahachchi , Lallindra Gooneratne , Anil Jayasekera , Dorota Muth-Pawlak , Robert Moulder , Susumu Y. Imanishi , Ari Ariyaratne , Anne Rokka , Garry L. Corthals","doi":"10.1016/j.euprot.2015.04.001","DOIUrl":null,"url":null,"abstract":"<div><p>More than 90% of polycythemia vera (PV) patients have a mutation in the protein JAK2, which is closely associated with the erythrocyte membrane. With the comparison of 1-D gels of erythrocyte membranes obtained from PV patients treated with hydroxycarbamide and those of untreated controls we observed significant differences in the region of 40–55<!--> <!-->kDa. On the basis of the LC–MS/MS analysis of this region we report up-regulation of four protein disulfide isomerases, which was subsequently confirmed by targeted mass spectrometric analysis. In further studies it will be prudent to compare this in patients both treated and not treated with hydroxycarbamide.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"7 ","pages":"Pages 43-53"},"PeriodicalIF":0.0000,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.04.001","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515000100","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 5
Abstract
More than 90% of polycythemia vera (PV) patients have a mutation in the protein JAK2, which is closely associated with the erythrocyte membrane. With the comparison of 1-D gels of erythrocyte membranes obtained from PV patients treated with hydroxycarbamide and those of untreated controls we observed significant differences in the region of 40–55 kDa. On the basis of the LC–MS/MS analysis of this region we report up-regulation of four protein disulfide isomerases, which was subsequently confirmed by targeted mass spectrometric analysis. In further studies it will be prudent to compare this in patients both treated and not treated with hydroxycarbamide.
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