Stefan Bugovsky , Wolfgang Winkler , Werner Balika , Günter Allmaier
{"title":"Long time storage (archiving) of peptide, protein and tryptic digest samples on disposable nano-coated polymer targets for MALDI MS","authors":"Stefan Bugovsky , Wolfgang Winkler , Werner Balika , Günter Allmaier","doi":"10.1016/j.euprot.2015.07.013","DOIUrl":null,"url":null,"abstract":"<div><p>Archiving of biological specimens is important due to the importance of reanalysis of already prepared MALDI MS samples or simultaneuous analysis of a sample series. Proteins/peptides and digests were prepared for measurement on a polymer-based metal nano-coated MALDI target and subjected to various storage conditions (−80<!--> <!-->°C to RT) in a vacuum-sealed pouch and at atmosphere for 6 months. The MS data gathered from these preparations illustrate trends in the aging of different samples and to find optimal storage conditions (−20 or −80<!--> <!-->°C in low oxygen environment). The disposable/low cost target proved to be a suitable platform for storage and MALDI MS.</p></div>","PeriodicalId":38260,"journal":{"name":"EuPA Open Proteomics","volume":"8 ","pages":"Pages 48-54"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.euprot.2015.07.013","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EuPA Open Proteomics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212968515300155","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 2
Abstract
Archiving of biological specimens is important due to the importance of reanalysis of already prepared MALDI MS samples or simultaneuous analysis of a sample series. Proteins/peptides and digests were prepared for measurement on a polymer-based metal nano-coated MALDI target and subjected to various storage conditions (−80 °C to RT) in a vacuum-sealed pouch and at atmosphere for 6 months. The MS data gathered from these preparations illustrate trends in the aging of different samples and to find optimal storage conditions (−20 or −80 °C in low oxygen environment). The disposable/low cost target proved to be a suitable platform for storage and MALDI MS.
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