Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Q4 Biochemistry, Genetics and Molecular Biology EuPA Open Proteomics Pub Date : 2015-09-01 DOI:10.1016/j.euprot.2015.06.001

Andrew J. Percy , Jessica Tamura-Wells , Juan Pablo Albar , Kerman Aloria , Ardeshir Amirkhani , Gabriel D.T. Araujo , Jesus M. Arizmendi , Francisco J. Blanco , Francesc Canals , Jin-Young Cho , Núria Colomé-Calls , Fernando J. Corrales , Gilberto Domont , Guadalupe Espadas , Patricia Fernandez-Puente , Concha Gil , Paul A. Haynes , Maria Luisa Hernáez , Jin Young Kim , Arthur Kopylov , Christoph H. Borchers

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引用次数: 29

Abstract

The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.

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仪器平台和实验工作流程的实验室间评估，用于定量准确性和可重复性评估

在一项涉及16个实验室和19个LC-MS /MS平台的大型国际研究中，使用两种用于评估仪器性能的试剂盒和一种用于评估整个自下而上工作流程的试剂盒，检查了血浆蛋白定量在实验室之间和仪器类型之间的可重复性。仪器类型对结果质量的影响较小，表明了同位素标记标准品LC/MRM-MS的稳健性。技术人员的技能是一个因素，因为样品制备中的错误和次优的LC-MS性能是显而易见的。这突出了在对患者样本进行定量之前进行适当培训和常规质量控制的重要性。

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