First study of complete genome of Dengue-3 virus from Rajasthan, India: genomic characterization, amino acid variations and phylogenetic analysis

Virology reports Pub Date : 2016-12-01 DOI:10.1016/j.virep.2016.05.003

Annette Angel, Bennet Angel, Ajay Prakash Joshi, Rajendra Kumar Baharia, Suman Rathore, Vinod Joshi

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引用次数: 8

Abstract

First study on the complete genome characterization of Dengue-3 virus is reported from dengue endemic state Rajasthan, India. The genome of 10,672 base pairs was studied with reference to global and the regional genomes of Dengue-3 virus. 388 variations were observed in the nucleotide sequences with reference to the NCBI genome, including 34 variations in the amino acids. Of these 34 AA variations, 4 variations showed AA substitution in Envelope proteins and 1 in Anchored capsid region. The remaining 29 variations were observed in amino acids constituting non structural (NS) proteins. The reported mutations, especially those leading to amino acid variations in non-structural proteins of virus may influence the clinical profile of patients and accuracy of ongoing diagnostic tests. Reported amino acid variations in virus envelope may influence the immune response of patients. Phylogenetic studies showed similarity of reported genome with genomes from India, Pakistan and China.

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印度拉贾斯坦邦登革热-3型病毒全基因组的首次研究:基因组特征、氨基酸变异和系统发育分析

印度登革热流行邦拉贾斯坦邦报道了首个关于登革热-3型病毒全基因组特征的研究。参考登革热-3型病毒全球和区域基因组，对10672个碱基对的基因组进行了研究。在参考NCBI基因组的核苷酸序列中观察到388个变异，其中包括34个氨基酸变异。在这34个AA变异中，4个变异在包膜蛋白中发生替换，1个在锚定衣壳区发生替换。其余29个变异是在构成非结构蛋白的氨基酸中观察到的。已报道的突变，特别是那些导致病毒非结构蛋白氨基酸变异的突变，可能影响患者的临床表现和正在进行的诊断测试的准确性。已报道的病毒包膜氨基酸变异可能影响患者的免疫反应。系统发育研究表明，所报道的基因组与来自印度、巴基斯坦和中国的基因组相似。

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