Genomic analysis of Sardinian 26544/OG10 isolate of African swine fever virus

Virology reports Pub Date : 2016-12-01 DOI:10.1016/j.virep.2016.09.001

Donatella Bacciu , Massimo Deligios , Giovanna Sanna , Maria Paola Madrau , Maria Luisa Sanna , Silvia Dei Giudici , Annalisa Oggiano

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引用次数: 11

Abstract

Comparative genomic analysis aims to underscore genetic assortment diversification in distinct viral isolates, to identify deletions and to carry out evolutionary studies. We sequenced the first complete genome of an ASFV p72 genotype I strain isolated from domestic pigs in Sardinia (Italy) using Next-Generation Sequence (NGS) technology. The genome is 182,906 bp long, contains 164 ORFs and has a 99.20% nucleotide identity to the L60 strain. Comparison analysis against the 16 ASFV genomes available in the database showed that 136 ORFs are present in nine ASFV isolates annotated to date. The most divergent ORFs codify for uncharacterized proteins such as X69R and DP96R, which have 51.3% and 70.4% nucleotide identity to the other isolates. A comparison between the Sardinian isolate and the avirulent isolates OURT 88/3, NHV, BA71V was also carried out. Major variations were found within the multigene families (MGFs) located in the left and right genome regions.

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非洲猪瘟病毒撒丁岛26544/OG10分离株基因组分析

比较基因组分析旨在强调不同病毒分离株的遗传分类多样化，确定缺失并进行进化研究。我们利用新一代测序技术(NGS)对意大利撒丁岛家猪分离的ASFV p72基因型1株进行了首次全基因组测序。该基因组长182,906 bp，包含164个orf，与L60菌株的核苷酸同源性为99.20%。与数据库中16个ASFV基因组的比较分析显示，迄今为止注释的9株ASFV分离株中存在136个orf。变异最大的orf编码的是未鉴定的蛋白，如X69R和DP96R，它们与其他分离株的核苷酸同源性分别为51.3%和70.4%。并将撒丁岛分离株与OURT 88/3、NHV、BA71V无毒分离株进行了比较。主要的变异是在位于左侧和右侧基因组区域的多基因家族(mgf)中发现的。

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