Newly characterized arboviruses of northern Australia

Bixing Huang , Richard Allcock , David Warrilow
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引用次数: 9

Abstract

Arboviruses circulate in the biota of northern Australia, sometimes causing disease in animals and humans. Five different arboviruses, isolated and stored for over 30 years, were re-cultured and sequenced using high-throughput sequencing technology (Ion Torrent PGM) for identification and genetic characterization. Phylogenetic analysis, primarily of sequence fragments of the large segment, indicated classification as members of the Family Bunyaviridae (Belmont, Little Sussex, Parker's Farm, and Thimiri viruses). Another was identified as a member of the Family Rhabdoviridae (Beatrice Hill virus) based on phylogenetic analysis of the RNA polymerase region.

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澳大利亚北部新发现的虫媒病毒
虫媒病毒在澳大利亚北部的生物群中传播,有时会引起动物和人类的疾病。对分离保存超过30年的5种虫媒病毒进行再培养,并利用高通量测序技术(Ion Torrent PGM)对其进行鉴定和遗传表征。系统发育分析,主要是对大片段的序列片段进行分析,表明分类为布尼亚病毒科成员(贝尔蒙特病毒、小苏塞克斯病毒、帕克农场病毒和蒂米里病毒)。根据RNA聚合酶区系统发育分析,另一株被鉴定为横纹肌病毒科(Beatrice Hill病毒)成员。
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