Ectomycorrhizal fungi and dark septate endophyte inoculation improve growth and tolerance of Pinus tabulaeformis under cadmium stress

Abstract

Forest trees can establish symbiotic associations with dark septate endophytes (DSEs) and ectomycorrhizal fungi (ECMF) simultaneously. However, the combined effects of these two fungi on the growth and cadmium (Cd) tolerance of host plants remain largely unexplored. To address this knowledge gap, a pot experiment was conducted to examine the effects of the interaction between an ECMF strain (Suillus granulatus) and a DSE strain (Pseudopyrenochaeta sp.) on Pinus tabulaeformis under Cd stress, by assessing plant growth and physiological parameters, nutrient uptake, and soil properties. Notably, the colonization rates of both fungal strains were found to increase in response to Cd stress, with the extent of this increase being influenced by the specific fungal species and the Cd level in the soil. Compared to the non-inoculation treatment, single inoculation with fungal strain resulted in enhanced biomass, root development, and nutrient contents in P. tabulaeformis seedlings under Cd stress. Furthermore, a synergistic effect was observed when these seedlings were co-inoculated with S. granulatus and Pseudopyrenochaeta sp., as indicated by significantly greater measurements in various indicators compared to both the single and non-inoculation treatments. Fungal inoculation effectively regulated the antioxidant defense responses and photosynthesis of P. tabulaeformis seedlings subjected to Cd stress, particularly in the co-inoculation treatment. In addition, fungal inoculation facilitated the Cd accumulation in P. tabulaeformis, suggesting a promising potential for the implementation of bioremediation strategies in the areas contaminated with heavy metals. The findings from this study indicate that the utilization of root symbiotic fungi obtained from stress environments could potentially enhance the growth performance and tolerance of P. tabulaeformis towards heavy metals, and co-inoculation of both fungal groups may result in even more pronounced synergistic effects on the overall fitness of the plant.