M. Steinitz, Ilkka Seppälä, K. Eichmann , George Klein
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引用次数: 22
Abstract
A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosaminecoupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 I-lg/ml anti-A-CHO antibodies of the μgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.
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