Lan QI , Wen-Quan WANG , Zhen-Wen ZHANG , Jian-Qiu YE , Kai-Mian LI
{"title":"DNA Fingerprints of 18 Cassava Varieties Based on Sequence-Related Amplified Polymorphism Markers","authors":"Lan QI , Wen-Quan WANG , Zhen-Wen ZHANG , Jian-Qiu YE , Kai-Mian LI","doi":"10.1016/S1875-2780(09)60076-6","DOIUrl":null,"url":null,"abstract":"<div><p>Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (<em>Manihot esculenta</em> Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.</p></div>","PeriodicalId":7085,"journal":{"name":"Acta Agronomica Sinica","volume":"36 10","pages":"Pages 1642-1648"},"PeriodicalIF":0.0000,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1875-2780(09)60076-6","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Agronomica Sinica","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875278009600766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 3
Abstract
Thirty-six pairs of polymorphic SRAP markers were used to construct DNA fingerprint of 18 cassava (Manihot esculenta Crantz) varieties. Among the total 320 amplified bands, 235 (73.4%) were polymorphic with an average of 6.5 bands per primer pair. The unweighted pair-group method with arithmetic average (UPGMA) cluster showed 4 major groups at similarity index of 0.734. Two primer combinations, Me1/Em5 and Me24/Em10, had the highest efficiency to distinguish cassava varieties, which were applicable to develop the DNA fingerprints of the 18 cassava varieties. The amplified bands were converted into binary codes with presence (1) and absence (0), and each variety had a unique 18-digital code. The confident probability of the DNA fingerprint was as high as 99.9996%.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
