Electrical stimulation promotes the wound-healing properties of diabetic human skin fibroblasts

IF 3.1 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Tissue Engineering and Regenerative Medicine Pub Date : 2022-04-20 DOI:10.1002/term.3305

Atieh Abedin-Do, Ze Zhang, Yvan Douville, Mireille Méthot, Julien Bernatchez, Mahmoud Rouabhia

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引用次数: 7

Abstract

This study evaluated the effect of low (20 and 40 mV/mm) intensities of electrical stimulation on the proliferation and migration of skin fibroblasts from diabetic donors. We also examined the effect of electrical stimulation on modulating the capacity of fibroblasts to contract collagen gel, express alpha-smooth muscle actin, and secrete proteolytic enzymes involved in regulating extracellular matrix synthesis and degradation. Our study shows that 20 and 40 mV/mm of stimulation increased the growth of fibroblasts extracted from diabetic patients but not from non-diabetic donors. Electrical stimulation increased the migration of diabetic fibroblasts, their capacity to contract collagen gel, and the expression of alpha-smooth muscle actin and promoted different proteolytic enzymes involved in accelerating wound healing. Overall results confirm the effectiveness of electrical stimulation in modulating the wound healing activities of fibroblasts extracted from diabetic skin donors. This study, therefore, suggests the possible use of electrical stimulation to promote diabetic foot ulcer healing by stimulating the wound healing properties of skin fibroblasts.

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电刺激促进糖尿病人皮肤成纤维细胞的伤口愈合特性

本研究评估了低强度(20和40 mV/mm)电刺激对糖尿病供体皮肤成纤维细胞增殖和迁移的影响。我们还研究了电刺激对成纤维细胞收缩胶原凝胶、表达α -平滑肌肌动蛋白和分泌参与调节细胞外基质合成和降解的蛋白水解酶的能力的影响。我们的研究表明，20和40 mV/mm的刺激能促进从糖尿病患者身上提取的成纤维细胞的生长，而不是从非糖尿病供体身上提取的成纤维细胞。电刺激增加了糖尿病成纤维细胞的迁移、收缩胶原凝胶的能力和α -平滑肌肌动蛋白的表达，并促进了参与加速伤口愈合的不同蛋白水解酶的表达。总体结果证实了电刺激在调节从糖尿病皮肤供体中提取的成纤维细胞的伤口愈合活性方面的有效性。因此，这项研究表明，电刺激可能通过刺激皮肤成纤维细胞的伤口愈合特性来促进糖尿病足溃疡的愈合。

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来源期刊

Journal of Tissue Engineering and Regenerative Medicine 生物-工程：生物医学

CiteScore

7.50

自引率

3.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Tissue Engineering and Regenerative Medicine publishes rapidly and rigorously peer-reviewed research papers, reviews, clinical case reports, perspectives, and short communications on topics relevant to the development of therapeutic approaches which combine stem or progenitor cells, biomaterials and scaffolds, growth factors and other bioactive agents, and their respective constructs. All papers should deal with research that has a direct or potential impact on the development of novel clinical approaches for the regeneration or repair of tissues and organs. The journal is multidisciplinary, covering the combination of the principles of life sciences and engineering in efforts to advance medicine and clinical strategies. The journal focuses on the use of cells, materials, and biochemical/mechanical factors in the development of biological functional substitutes that restore, maintain, or improve tissue or organ function. The journal publishes research on any tissue or organ and covers all key aspects of the field, including the development of new biomaterials and processing of scaffolds; the use of different types of cells (mainly stem and progenitor cells) and their culture in specific bioreactors; studies in relevant animal models; and clinical trials in human patients performed under strict regulatory and ethical frameworks. Manuscripts describing the use of advanced methods for the characterization of engineered tissues are also of special interest to the journal readership.