Cell Proliferation Detected with Flow Cytometric Cell Cycle Analysis and Immunohistochemical Detection of Proliferating Cell Nuclear Antigen (PCNA) from Somatic Tissues of Eastern Oysters, Crassostrea virginica ∗

Environmental bioindicators Pub Date : 2006-12-12 DOI:10.1080/15555270600868174

J. Jenkins, J. Lapeyre

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引用次数: 1

Abstract

Two novel biomarker of response assays were developed and compared for use with bivalves. Bivalve mollusks are often used as bioindicators to monitor contaminant body burdens and are employed globally in pollution monitoring and as sentinels of environmental quality. The prevalence of proliferating cells in tissues of the eastern oyster, Crassostrea virginica, (n = 10) was investigated by using immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and by flow cytometric analysis of DNA in cell cycle phases. The PCNA protein is highly conserved and central to DNA synthesis in dividing cells. This protein was detected by using a commercial antibody in the histological staining of gills, mantle, digestive gland, stomach, and labial palps. Cells obtained from these same organs and from the hearts of the oysters were used for flow cytometry (FCM). The proportions of nuclei in the S plus G2/M and G2/M fractions were compared with percentages of PCNA-positive cells scored through microscopy. ...

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流式细胞术细胞周期分析和免疫组化检测东方牡蛎体细胞组织增殖细胞核抗原(PCNA)的研究

开发了两种新的生物标志物反应测定方法，并将其用于双壳类动物进行了比较。双壳类软体动物经常被用作监测污染物体负荷的生物指标，在全球范围内被用于污染监测和环境质量的哨兵。采用增殖细胞核抗原(PCNA)免疫组化染色和细胞周期DNA流式细胞术分析，研究了东牡蛎(Crassostrea virginica, n = 10)组织中增殖细胞的流行情况。PCNA蛋白是高度保守的，是分裂细胞中DNA合成的核心。该蛋白通过商业抗体在鳃、套、消化腺、胃和唇瓣的组织学染色中检测到。从这些器官和牡蛎心脏中获得的细胞用于流式细胞术(FCM)。将S + G2/M和G2/M组中细胞核的比例与显微镜下pcna阳性细胞的百分比. ...进行比较

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Environmental bioindicators

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