Cytochrome P450-Dependent Mixed Function Oxidases (MFO) System Dynamics During the Poly Aromatic Hydrocarbon (PAH) Metabolism in Green Mussel Perna Viridis (Linnaeus, 1758)

Abstract

Polyaromatic hydrocarbons (PAH's) are the prominent and most common pollutants in aquatic environments, particularly in marine water. The discharge of hydrocarbons into the sea might be of great concern for marine species living close to dumping sites. Therefore, toxicological properties of hydrocarbons released into marine environments need to be evaluated. PAH pollution potential may be predicted by assessing the induction of hepatic cytochrome P450-associated enzyme activity. The inducibility and activity of phase I reduction nicotinamide adenine dinucleotide phosphate, reduced (NADPH) cytochrome c reductase (CCR), cytochrome c oxidase (COX) and three CYP450 isoforms (benzyloxyresorufin - O-dealkylase [BROD], ethoxyresorufin-O-dealkylase [EROD] and methoxyresorufin-O-dealkylase [MROD] ) enzymes were measured in the hepatic S9 fraction prepared from Perna viridis collected from three sites: a highly oil-polluted site (Kasimedu fishing harbor, Rayapuram, Chennai [Station1]); a moderately polluted off-sho...