Thermostable lipase from novel Pseudomonas sp. VITSDVM1 isolated from bovine milk

Q1 Biochemistry, Genetics and Molecular Biology Frontiers in Life Science Pub Date : 2015-03-06 DOI:10.1080/21553769.2015.1015057

C. Subathra Devi, V. Mohanasrinivasan, Mathur Chetna, Anil Sase Nikhil, S. Jemimah Naine

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引用次数: 6

Abstract

The main aim of this study was to identify lipase-producing bacteria from bovine milk samples. The strain possessing the highest lipase activity was identified by physiological and molecular characterization. 16S rRNA phylogenetic analysis showed highest similarity with Pseudomonas sp. and the strain was named Pseudomonas sp. VITSDVM1. The lipase productivity was improved by varying the utilization of substrates, i.e. banana and potato peel extracts, in the production medium. The results of optimization studies showed that lipase activity was maximum with groundnut oil, compared with the other carbon sources used, and with peptone as a nitrogen source. The optimum pH and temperature were 8 and 40°C, respectively. The optimum substrate concentration was 20% for the incubation period of 72 h with a maximum activity of 4.66 U mL−1. The purified lipase enzyme analyzed by high-performance liquid chromatography revealed a retention time of 3.6 min. Hence, lipase production using agricultural waste such as banana and potato peels could be used in industrial applications.

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从牛乳中分离的新型假单胞菌属VITSDVM1耐热脂肪酶

本研究的主要目的是从牛乳样品中鉴定产生脂肪酶的细菌。对脂肪酶活性最高的菌株进行了生理和分子鉴定。16S rRNA系统发育分析结果显示该菌株与假单胞菌属相似性最高，并将其命名为Pseudomonas sp. VITSDVM1。通过改变生产培养基中底物(即香蕉和马铃薯皮提取物)的利用率，提高了脂肪酶的产量。优化研究结果表明，与其他碳源相比，以花生油和蛋白胨为氮源的脂肪酶活性最高。最适pH为8℃，最适温度为40℃。最适底物浓度为20%，孵育72 h，最大活性为4.66 U mL−1。纯化后的脂肪酶经高效液相色谱分析，酶保留时间为3.6 min。因此，利用香蕉和土豆皮等农业废弃物生产脂肪酶可用于工业应用。

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Frontiers in Life Science MULTIDISCIPLINARY SCIENCES-

CiteScore

5.50

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0.00%

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期刊介绍： Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.