Eugenol, an essential oil of clove, causes disruption to the cell wall of Candida albicans (ATCC 14053)

Q1 Biochemistry, Genetics and Molecular Biology Frontiers in Life Science Pub Date : 2015-07-03 DOI:10.1080/21553769.2015.1045628

B. Latifah-Munirah, W. H. Himratul-Aznita, N. M. Mohd Zain

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引用次数: 35

Abstract

The aim of this study was to determine the effect of eugenol on the integrity of the cell wall of Candida albicans. Minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and percentage inhibition of diameter growth (PIDG) of eugenol on C. albicans (ATCC 14053) were determined. The effect of eugenol on the growth profile was also evaluated. The release of cellular material and changes in cell permeability, including ultrastructural alterations to the morphology, were assessed using scanning electron microscopy, and surface disruption to the cell wall structure of C. albicans by atomic force microscopy. The MIC and MFC values of eugenol were found to be 1.0% v/v, while the PIDG was dose dependent. Eugenol influenced cell growth and was fungicidal towards C. albicans. Eugenol was also found to encourage cell leakage, causing the release of cellular material, and to increase cell permeability. The ultrastructure and cell surface morphology were also altered by the presence of eugenol. Thus, eugenol was found to disrupt the cell wall of C. albicans.

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丁香酚是丁香的一种精油，能破坏白色念珠菌的细胞壁(ATCC 14053)。

本研究的目的是确定丁香酚对白色念珠菌细胞壁完整性的影响。测定丁香酚对白色念珠菌(ATCC 14053)的最小抑菌浓度(MIC)、最小杀真菌浓度(MFC)和直径生长抑制率(PIDG)。并对丁香酚对其生长的影响进行了评价。利用扫描电镜观察细胞物质的释放和细胞通透性的变化，包括形态的超微结构改变，利用原子力显微镜观察白色念珠菌细胞壁结构的表面破坏。丁香酚的MIC和MFC值为1.0% v/v, PIDG呈剂量依赖性。丁香酚影响细胞生长，对白色念珠菌有杀真菌作用。丁香酚还被发现可以促进细胞渗漏，导致细胞物质的释放，并增加细胞的渗透性。丁香酚的存在也改变了细胞的超微结构和表面形态。因此，丁香酚被发现破坏白色念珠菌的细胞壁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Life Science MULTIDISCIPLINARY SCIENCES-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.