Immune response to 17beta-estradiol involved in polymer gels: antigen specificity and affinity of hybridoma clones.

A. Başalp, Z. Mustafaeva, M. Mustafaev, E. Bermek
{"title":"Immune response to 17beta-estradiol involved in polymer gels: antigen specificity and affinity of hybridoma clones.","authors":"A. Başalp, Z. Mustafaeva, M. Mustafaev, E. Bermek","doi":"10.1089/027245700750053995","DOIUrl":null,"url":null,"abstract":"The immunogenic properties of 17beta-estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17beta-estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with Kd values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"19 6 1","pages":"495-9"},"PeriodicalIF":0.0000,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245700750053995","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hybridoma","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/027245700750053995","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10

Abstract

The immunogenic properties of 17beta-estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17beta-estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with Kd values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
聚合物凝胶对17 -雌二醇的免疫反应:杂交瘤克隆的抗原特异性和亲和力。
研究了用带负电荷的聚合物凝胶固定17β -雌二醇的免疫原性,并分析了产生抗体的特异性。所制备的聚合物凝胶由疏水的雌二醇核组成,周围有亲水的聚阴离子作为电晕。作为一种免疫原,它被认为通过双重模式起作用,即作为半抗原递送系统(延长效应)和作为多电解质佐剂。含有雌二醇的聚合物凝胶即使不添加传统佐剂也具有较高的雌二醇特异性免疫原性。对聚合物凝胶中捕获的雌二醇与与牛血清白蛋白(BSA.E) +不完全弗氏佐剂(IFA)结合的雌二醇的比较研究显示,在诱导特异性抗体方面,雌二醇具有相似的免疫原性。通过将17 β -雌二醇固定在聚合物凝胶中进行短期免疫,我们开发了10种特异性单克隆抗体,其Kd值在1.2 X 10(-7)和8 X 10(-8) M之间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
相关文献
Respiratory disease in people with bipolar disorder: a systematic review and meta-analysis
IF 9.6 1区 医学Molecular PsychiatryPub Date : 2024-11-14 DOI: 10.1038/s41380-024-02793-1
David Laguna-Muñoz, Ana Jiménez-Peinado, María José Jaén-Moreno, Cristina Camacho-Rodríguez, Gloria Isabel del Pozo, Eduard Vieta, Javier Caballero-Villarraso, Muhammad Ijlal Khan, Fernando Rico-Villademoros, Fernando Sarramea
Factors Associated With Physical Activity and Sedentary Behavior in People With Parkinson Disease: A Systematic Review and Meta-Analysis.
IF 3.5 4区 医学Physical TherapyPub Date : 2024-12-06 DOI: 10.1093/ptj/pzae114
Vitoria L Domingues, Marina P Makhoul, Tatiana B de Freitas, Janaine C Polese, Carla Silva-Batista, Fabio A Barbieri, Camila Torriani-Pasin
Muscle Strength and Power in People With Parkinson Disease: A Systematic Review and Meta-analysis.
IF 3.8 3区 医学Journal of Neurologic Physical TherapyPub Date : 2023-01-01 DOI: 10.1097/NPT.0000000000000421
Mads Gamborg, Lars Grøndahl Hvid, Cecilie Thrue, Sverker Johansson, Erika Franzén, Ulrik Dalgas, Martin Langeskov-Christensen
来源期刊
Hybridoma
Hybridoma 医学-免疫学
自引率
0.00%
发文量
0
审稿时长
4-8 weeks
期刊最新文献
Monoclonal antibody hTG4-Ma6 against human transglutaminase 4 Monoclonal antibodies 6D2 and 8H3 against mouse Chd2 Monoclonal antibody 8E3 against mouse Dhx9/NDHII/RHA Monoclonal antibodies 2F12H4 and 3C3F1 against mouse Pax7 Monoclonal antibody 1H7A10 against mouse Brm
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1