Use of molecular scatology to assess the diet of feral cats living in urban colonies

Abstract

The overpopulation of domestic cats (Felis catus) presents a serious concern for wildlife conservationists, animal welfare advocates, public health officials, and community members alike. In cities, free-ranging, unowned cats often form high-density groups (commonly called ‘colonies’) around human provisioned food sources. While previous diet studies have primarily utilized morphology-based methods, molecular techniques offer a higher resolution alternative. In this study, we used next-generation sequencing techniques to examine the diet composition of feral cats living in five Trap-Neuter-Return colonies located in urban parks on Staten Island, a borough of New York City. We hypothesized that (1) cats living in urban colonies would still consume natural prey despite being regularly fed and (2) that the composition of taxa represented in the diet of each colony would vary, possibly due to differences in prey availability across sites. In total, 16 vertebrate prey taxa were identified in the diet, 13 at the genus level and 3 at the family level. Despite being regularly fed, 58.2% of cat scats contained DNA from natural prey. The diet composition of the cat colonies differed depending on the land cover composition surrounding the colony with the frequency of native prey positively correlated with the proportion of green space and that of non-native prey with developed land cover types. The use of molecular techniques combined with environmental DNA methods offers a promising, non-invasive approach to assessing the diet and consequently, impact of a highly abundant and non-native predator on the persistence of wildlife communities in cities.