Affinity of adherence in vitro and colonization of mice intestine by enterotoxigenic Escherichia coli (ETEC)

IF 2.2 4区生物学 Q3 MICROBIOLOGY Fems Microbiology Letters Pub Date : 1984-06-01 DOI:10.1111/j.1574-6968.1984.tb01043.x

A. Zilberberg, I. Ofek, J. Goldhar

引用次数: 6

Abstract

Abstract The correlation between affinity of adherence in vitro to mice intestinal segments and infectivity in vivo was examined in an enterotoxigenic Escherichia coli (ETEC) strain. Two unstable phenotypes of the same strain were obtained by growing bacteria in agar or broth. Affinity of adherence in vitro calculated by Scatchard plot analysis of agar-grown bacteria was significantly higher than that of broth-grown bacteria. The effective dose of infection of agar-grown bacteria at 3, 24 and 72 h after infection, was one-tenth, one-half and the same, respectively, of that of broth-grown bacteria. The results suggest that the differences in the adhering ability of the inoculum influenced the number of bacteria retained in the intestine during the early phases of infection.

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产肠毒素大肠杆菌(ETEC)体外粘附和小鼠肠道定植的亲和力

摘要采用产肠毒素大肠杆菌(ETEC)研究了体外粘附小鼠肠段的亲和力与体内感染性的关系。在琼脂或肉汤中培养细菌获得了同一菌株的两种不稳定表型。通过Scatchard图分析计算，琼脂培养菌的体外黏附亲和力显著高于肉汤培养菌。感染后3、24和72 h，琼脂培养细菌的有效感染剂量分别为肉汁培养细菌的1 / 10、1 / 2和1 / 2。结果表明，接种物粘附能力的差异影响了感染早期肠道中保留的细菌数量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.