HPLC COUPLED WITH ION TRAP MS/MS FOR ANALYSIS OF TERTIARY BUTYLHYDROQUINONE IN EDIBLE OIL SAMPLES

Journal of Food Lipids Pub Date : 2008-02-05 DOI:10.1111/j.1745-4522.2007.00098.x

WEN HUANG, HAI NIU, BI SHI, MINLI YANG, JIXIN JIANG

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引用次数: 11

Abstract

ABSTRACT

A rapid, sensitive, precise and accurate quantitative liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the measurement of tertiary butylhydroquinone (TBHQ) in commercial edible oil was developed and validated. After extraction, samples without prior concentration were analyzed by ion trap MS/MS in MS and MS/MS negative ion detection modes using electrospray ionization. The method showed satisfactory recovery (81.9 ± 1.9–109.6 ± 4.6%), sensitivity (lower limit of quantitation of 48 µg/L for TBHQ) and precision (≤5.2%), as well as excellent linearity (R² ≥ 0.99), with a run time of only 5 min. The method was successfully applied to the determination of TBHQ in commercial samples of edible oils containing various natural antioxidants, providing a promising and convenient entry to monitor the use of TBHQ.

PRACTICAL APPLICATIONS

HPLC coupled with the ion trap tandem mass spectrometry method with high selectivity, sensitivity, precision and accuracy is suitable for routine quality control analysis of tertiary butylhydroquinone (TBHQ) in edible oils. Analyzing each oil sample by this method takes only 5 min. Moreover, the evaluation of TBHQ in commercial edible oil by this method does not suffer from interference of some coexisting potential peculiar chemical components.

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高效液相色谱-离子阱-质谱法分析食用油中叔丁基对苯二酚

建立了一种快速、灵敏、精确、准确的液相色谱/串联质谱(LC/MS/MS)定量测定商品食用油中叔丁基对苯二酚(TBHQ)含量的方法，并进行了验证。提取后，采用离子阱质谱/质谱分析，采用电喷雾电离的MS/MS和MS/MS负离子检测模式。该方法回收率(81.9±1.9 ~ 109.6±4.6%)、灵敏度(TBHQ定量下限为48µg/L)、精密度(≤5.2%)良好，线性良好(R2≥0.99)，运行时间仅为5 min。该方法成功地应用于含有多种天然抗氧化剂的食用油商业样品中三丁基六氢胺的测定，为监测三丁基六氢胺的使用提供了一个有前途和方便的途径。高效液相色谱-离子阱串联质谱法具有较高的选择性、灵敏度、精密度和准确度，适用于食用油中叔丁基对苯二酚(TBHQ)的常规质量控制分析。用这种方法分析每个油样只需要5分钟。此外，利用该方法对商品食用油中四氢大麻酚的评价不受一些共存的潜在特殊化学成分的干扰。

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Journal of Food Lipids 工程技术-食品科技

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