Tracking GFP-labeled Transplanted Mouse MSC in Nude Mice Using in Vivo Fluorescence Imaging

Q4 Engineering Nano Biomedicine Pub Date : 2014-01-01 DOI:10.11344/NANO.6.73

M. Taira, Wataru Hatakeyama, Jun Yokota, N. Chosa, A. Ishisaki, Kyoko Takafuji, Hidemichi Kihara, H. Kondo, Masayuki Hattori

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引用次数: 1

Abstract

73 Introduction Stem cell therapy is now considered as a new therapeutic method to restore damaged organ including injured liver [1]. One important source of stem cells is mesenchymal stem cells (MSC) [2]. MSC can be easily collected from bone marrows of patients [3]. MSC is chemotactic to damaged organs and tissues which often secret cytokines and chemokines [4]. Subsequently, MSC can settle and multiply in the damaged zones (namely, by homing phenomenon), and often heal the damaged or inflamed organ and tissues [5]. This phenomenon has, however, not well been understood, yet. The fluorescent nature of the cells from green fluorescent protein (GFP)-transgenic mice facilitate the use in many kinds of cell transplantation experiments [6]. Immunitycompromised mice (nude mice) have been used as host animal so that the relation between transplanted GFP-transgenic mouse cells and host nude mouse body could be clarified [7]. The purpose of this study was, therefore, to monitor the fate of GFP-labeled transgenic Tracking GFP-labeled Transplanted Mouse MSC in Nude Mice Using in Vivo Fluorescence Imaging

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利用体内荧光成像技术追踪gfp标记的裸鼠移植小鼠间充质干细胞

干细胞治疗目前被认为是修复受损器官的一种新的治疗方法，包括损伤的肝细胞。干细胞的一个重要来源是间充质干细胞(MSC)[2]。骨髓间充质干细胞可以很容易地从患者骨髓中收集到。骨髓间充质干细胞对受损器官和组织具有趋化作用，这些器官和组织经常分泌细胞因子和趋化因子[4]。随后，MSC可以在受损区域(即通过归巢现象)定居和繁殖，并经常愈合受损或发炎的器官和组织[5]。然而，这种现象还没有被很好地理解。绿色荧光蛋白(GFP)转基因小鼠细胞的荧光特性为多种细胞移植实验提供了便利。利用免疫受损小鼠(裸鼠)作为宿主动物，阐明移植gfp转基因小鼠细胞与宿主裸鼠体之间的关系[10]。因此，本研究的目的是利用体内荧光成像技术监测gfp标记的转基因跟踪gfp标记的移植小鼠MSC在裸鼠中的命运

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来源期刊

Nano Biomedicine Engineering-Biomedical Engineering

CiteScore

0.30

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0.00%

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