Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes

H. Dwivedi, Kusum Agrawal, S. Saraf
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引用次数: 2

Abstract

Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium.
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筛选的野生/天然微生物产尿酸酶影响因素的评价
痛风是一种代谢紊乱,由于尿酸结晶沉积在关节或关节周围组织。尿酸酶(尿酸氧化酶)催化水溶性较低的尿酸(7毫克/分升)氧化为水溶性较强的化合物尿囊素(40℃时为11克/升),从而使尿酸易于排泄。本研究旨在建立一种新的产尿酸酶微生物筛选及产尿酸酶评价方法。这是通过利用尿酸在被尿酸酶作用时溶解的事实来实现的。该方法是一种新颖、廉价、简便、灵敏的尿酸酶筛选和评价方法。研究了产尿酸酶微生物在不同生长阶段的生物量和产尿酸酶曲线。采用Placket Burman统计设计研究了不同培养基成分对微生物产尿酸酶的影响。在营养培养基中添加尿酸被发现可以有效地增加培养基中生长的微生物的尿酸酶产量。
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来源期刊
E-journal of Chemistry
E-journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
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审稿时长
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