Glutamic acid decarboxylase in the rat ampullary cristae: immunocytochemical and immunoblotting studies

Primary sensory neuron : the international interdisciplinary journal reporting basic and clinical research on sensory receptors and primary afferent neurons Pub Date : 1998-01-01 DOI:10.1163/092996398750132142

G. Meza, Jang-Yen Wu, H. Zepeda, C. José

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Abstract

In order to investigate a putative neurotransmitter function of γ-aminobutyric acid (GABA) in the rat vestibule we chose to study the cellular localization and properties of glutamic acid decarboxylase (GAD), the rate-limiting enzyme for the synthesis of GABA, in the rat ampullary cristae, with respective immunocytochemical and immunoblotting techniques, using an extensively characterized rabbit anti-GAD serum. With these procedures we found a GAD-like immunopositivity in the sensory cells and in fibers of the crista ampullaris stroma. In ampullary cristae homogenates, a GAD composed of at least two subunits (53 and 67 kDa which were present also in rat brain and cerebellum homogenates) was encountered. These findings suggest that GAD present in the rat vestibule is homologous to the brain enzyme and it has the appropriate localization to synthesize GABA to be used as a neurotransmitter in the rat vestibule.

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大鼠壶腹嵴谷氨酸脱羧酶:免疫细胞化学和免疫印迹研究

为了研究γ-氨基丁酸(GABA)在大鼠前庭中的神经递质功能，我们选择利用免疫细胞化学和免疫印迹技术，研究大鼠壶腹嵴中合成GABA的限速酶谷氨酸脱羧酶(GAD)的细胞定位和性质，使用广泛表征的兔抗GAD血清。通过这些程序，我们发现壶腹嵴间质感觉细胞和纤维中的gad样免疫阳性。在壶腹嵴匀浆中，发现了至少由两个亚基(53和67 kDa)组成的GAD，它们也存在于大鼠脑和小脑匀浆中。这些发现表明，GAD存在于大鼠前庭中，与脑酶同源，并且在大鼠前庭中具有合适的定位，可以合成GABA作为神经递质。

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Primary sensory neuron : the international interdisciplinary journal reporting basic and clinical research on sensory receptors and primary afferent neurons

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