Assessment of Neuroprotective Properties of Melissa officinalis in Combination With Human Umbilical Cord Blood Stem Cells After Spinal Cord Injury.

IF 3.9 4区医学 Q2 NEUROSCIENCES ASN NEURO Pub Date : 2016-11-03 Print Date: 2016-10-01 DOI:10.1177/1759091416674833

Seyed Ruhollah Hosseini, Gholamreza Kaka, Mohammad Taghi Joghataei, Mehdi Hooshmandi, Seyed Homayoon Sadraie, Kayvan Yaghoobi, Alireza Mohammadi

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Abstract

Introduction: The pathophysiology of spinal cord injury (SCI) has a classically bad prognosis. It has been demonstrated that human umbilical cord blood stem cells (hUCBSCs) and Melissa officinalis (MO) are useful for the prevention of neurological disease.

Methods: Thirty-six adult male rats were randomly divided into intact, sham, control (SCI), MO, hUCBSC, and MO-hUCBSC groups. Intraperitoneal injection of MO (150 mg/kg) was commenced 24 hr post-SCI and continued once a day for 14 days. Intraspinal grafting of hUCBSCs was commenced immediately in the next day. The motor and sensory functions of all animals were evaluated once a week after the commencement of SCI. Electromyography (EMG) was performed in the last day in order to measure the recruitment index. Immunohistochemistry, reverse transcription-polymerase chain reaction, and transmission electron microscopy evaluations were performed to determine the level of astrogliosis and myelination.

Results: The results revealed that motor function (MO-hUCBSC: 15 ± 0.3, SCI: 8.2 ± 0.37, p < .001), sensory function (MO-hUCBSC: 3.57 ± 0.19, SCI: 6.38 ± 0.23, p < .001), and EMG recruitment index (MO-hUCBSC: 3.71 ± 0.18, SCI: 1.6 ± 0.1, p < .001) were significantly improved in the MO-hUCBSC group compared with SCI group. Mean cavity area (MO-hUCBSC: 0.03 ± 0.03, SCI: 0.07 ± 0.004, p < .001) was reduced and loss of lower motor neurons (MO-hUCBSC: 7.6 ± 0.43, SCI: 3 ± 0.12, p < .001) and astrogliosis density (MO-hUCBSC: 3.1 ± 0.15, SCI: 6.25 ± 1.42, p < 0.001) in the ventral horn of spinal cord were prevented in MO-hUCBSC group compared with SCI group.

Conclusion: The results revealed that the combination of MO and hUCBSCs in comparison with the control group has neuroprotective effects in SCI.

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脊髓损伤后香蜂草与人脐带血干细胞结合的神经保护特性评估

导言：脊髓损伤（SCI）的病理生理学通常预后不良。研究表明，人脐血干细胞（hUCBSCs）和香蜂草（MO）可用于预防神经系统疾病：方法：将36只成年雄性大鼠随机分为完整组、假组、对照组（SCI）、MO组、hUCBSC组和MO-hUCBSC组。SCI 后 24 小时开始腹腔注射 MO（150 毫克/千克），每天一次，持续 14 天。第二天立即开始椎管内移植 hUCBSC。所有动物的运动和感觉功能在 SCI 开始后每周评估一次。最后一天进行肌电图（EMG）检查，以测量募集指数。免疫组化、反转录聚合酶链式反应和透射电子显微镜评估是为了确定星形胶质细胞和髓鞘化的水平：结果显示，运动功能（MO-hUCBSC：15±0.3，SCI：8.2±0.37，P 结论：MO-hUCBSC与SCI联合治疗可显著改善运动功能：结果显示，与对照组相比，MO 和 hUCBSCs 的组合对 SCI 具有神经保护作用。

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来源期刊

ASN NEURO NEUROSCIENCES-

CiteScore

7.70

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： ASN NEURO is an open access, peer-reviewed journal uniquely positioned to provide investigators with the most recent advances across the breadth of the cellular and molecular neurosciences. The official journal of the American Society for Neurochemistry, ASN NEURO is dedicated to the promotion, support, and facilitation of communication among cellular and molecular neuroscientists of all specializations.