Screening of fungi producing ligninolytic enzymes

Q3 Agricultural and Biological Sciences Asia-Pacific Journal of Science and Technology Pub Date : 2016-07-15 DOI:10.14456/KKURJ.2016.39
Zecarias Ghebreslasie, D. Premjet, Siripong Permjet
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引用次数: 5

Abstract

A total of 61 fungi isolate were subjected for screening of ligninolytic enzyme by dye decolorization plate test. The results presented the diversity of 8 orders belonging to 17  families, 30 genera and 25 unidentified cultures. Seven families of Agaricales, three families of Polyporales, two families of Russulales and one family represent for each of  the orders, Boletales, Cantharellales, Phallales, Tremellales and Xylariales. The plate test perceived seven categorical groups as: strains decolorizing a single dye implies for  secreting only one of the three main enzymes ; a) Phenol red for LiP, b) Azure B for MnP c) RBBR for Lacc; strains decolorizing two of the organic colors relays for secreting only two enzymes as; d) Phenol red and Azure B, e) Phenol red and RBBR, f) Azure B and RBBR; and g) strains which decolorize three organic dyes produce three of the ligninolytic enzymes.
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产木质素分解酶真菌的筛选
采用染料脱色平板法对61株真菌分离物进行木质素降解酶的筛选。结果表明,该物种有8目,隶属于17科30属,25种未确定的文化。Agaricales有七个科,Polyporales有三个科,Russulales有两个科,Boletales、Cantharellales、Phallales、Tremellales和Xylariales各有一个科。平板试验认为七个分类组:菌株脱色单一染料意味着只分泌三种主要酶中的一种;a)酚红表示LiP, b)青蓝色b表示MnP, c) RBBR表示Lacc;对两种有机颜色进行脱色的菌株只分泌两种酶;d)酚红与天青B, e)酚红与RBBR, f)天青B与RBBR;g)脱色三种有机染料的菌株产生三种木质素分解酶。
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来源期刊
Asia-Pacific Journal of Science and Technology
Asia-Pacific Journal of Science and Technology Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
0.90
自引率
0.00%
发文量
0
审稿时长
8 weeks
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