Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of japonica rice 'Sagabiyori'.

IF 2 4区农林科学 Q2 AGRONOMY Breeding Science Pub Date : 2023-09-01 Epub Date: 2023-09-09 DOI:10.1270/jsbbs.23017

Saw Bo Day Shar, Cuong Dinh Nguyen, Sachiyo Sanada-Morimura, Hideshi Yasui, Shao-Hui Zheng, Daisuke Fujita

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Abstract

The brown planthopper (BPH: Nilaparvata lugens Stål) is one of the most destructive insects in rice production. The use of host plant resistance has potential to reduce damage caused by BPH. The heat tolerance japonica rice 'Sagabiyori', with superior grain quality and high soluble starch in the stem, is highly susceptible to damage by BPH. Here, to enhance its BPH resistance, we developed seven near-isogenic lines (NILs) carrying BPH2, BPH17-ptb, BPH32, BPH3, BPH17, BPH20, and BPH21 through marker-assisted selection and evaluated resistance to two BPH populations. Most lines were more resistant to the Hadano-1966 BPH population than Sagabiyori but were less effective against the highly virulent Koshi-2013 population. Nevertheless, in antixenosis tests, Koshi-2013 settled less on all NILs than on Sagabiyori. In addition, adult mortality and the percentage of fresh weight loss of lines carrying BPH17 and BPH3 indicated that these lines have higher resistance to Koshi-2013 than Sagabiyori. Current study revealed that BPH resistance of Sagabiyori became stronger by transferring BPH3 and BPH17 genes. Thus, BPH3 and BPH17 might be valuable for breeding programs to enhance BPH resistance of high grain quality rice varieties with heat tolerance.

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在粳稻 "Sagabiyori "的遗传背景中开发抗褐飞虱基因的近等基因系并确定其特征。

褐飞虱（BPH：Nilaparvata lugens Stål）是水稻生产中最具破坏性的昆虫之一。利用寄主植物的抗性有可能减少褐飞虱的危害。耐热性粳稻'Sagabiyori'的谷粒质量上乘，茎秆中可溶性淀粉含量高，但极易受到 BPH 的危害。在此，为了增强其对 BPH 的抗性，我们通过标记辅助选择技术培育了 7 个携带 BPH2、BPH17-ptb、BPH32、BPH3、BPH17、BPH20 和 BPH21 的近等基因系（NIL），并评估了其对两个 BPH 群体的抗性。大多数品系对 Hadano-1966 BPH 群体的抗性强于 Sagabiyori，但对高致病性的 Koshi-2013 群体的抗性较弱。尽管如此，在抗赤霉病试验中，Koshi-2013 在所有 NIL 上的定居率都低于 Sagabiyori。此外，携带 BPH17 和 BPH3 的品系的成虫死亡率和鲜重损失百分比表明，这些品系对 Koshi-2013 的抗性高于 Sagabiyori。目前的研究表明，转入 BPH3 和 BPH17 基因后，Sagabiyori 的抗 BPH 能力更强。因此，BPH3 和 BPH17 可能对育种计划很有价值，可用于提高具有耐热性的优质水稻品种对 BPH 的抗性。

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来源期刊

Breeding Science 农林科学-农艺学

CiteScore

4.90

自引率

4.20%

发文量

审稿时长

1.5 months

期刊介绍： Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews related to breeding. Research Papers are standard original articles. Notes report new cultivars, breeding lines, germplasms, genetic stocks, mapping populations, database, software, and techniques significant and useful for breeding. Reviews summarize recent and historical events related breeding. Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.