1H, 13C, and 15N Resonance Assignments of the DNA Binding Domain of Ler from Enteropathogenic E. coli in Complex with DNA

Abstract

Ler is a master regulator for the gene regulation of the locus of enterocyte effacement (LEE) pathogenicity island (PAI), which could activate the transcription of LEE2 to LEE5 by counteracting the repression of H-NS. Ler contains an N-terminal oligomerization domain, a linker region, and a C-terminal DNA binding domain. However, the DNA binding mechanism of Ler remains unclear. Here, we report the ¹H, ¹³C, and ¹⁵N NMR assignments of Ler^CTD/3A3T-DNA complex. We have achieved 97.8% completeness for the backbone and side-chain resonance assignments of Ler^CTD in the complex, excluding ¹³C′ resonances. In addition, about 91.5% of the ¹H resonances of 3A3T-DNA have been assigned. Our near complete assignments of this complex will significantly advance the parsing of the intermolecular NOE signals between Ler ^CTD and 3A3T-DNA. Therefore, our NMR assignments formed a good basis for further DNA binding mechanism studies of Ler.