Screening of bioactive compounds for biomedical and industrial uses from Actinobacteria isolated from the Parsik Cave (Turkey)

Abstract

Health issue and pollution of the environment are mainly caused by using chemicals and synthetic materials. This issue incites scientists to research for new biological compounds beneficial to human being. Caves, being extreme environments might be potential sources of these compounds. Actinobacteria, one of the main groups that are colonizing these environments, are known to contribute most of natural bioactive compounds. To investigate the potential uses of Parsik Cave Actinobacteria, identification of this group of isolates and the investigation of their secreted biological compounds constituted the principal aim of our study. The identification was achieved by sequencing 16S rRNA genes of 41 selected bacteria in which 28 species were identified as Actinobacteria. Microbacterium (21%), Pseudoarthrobacter (14%) were the most identified Actinobacteria genera. Antimicrobial effects of the isolates P1 and P16 were observed against standard microorganisms like Candida albicans. The GC-MS analysis of their broth shown antimicrobials, antioxidants, anticancer, and unknown compounds. PKS and NRPS were amplified respectively in 32.1 % and 53.5% of the identified Actinobacteria while their 25% have been both NRPS and PKS amplified. Amylase, gelatinase, cellulase, DNase, urease, and casein hydrolysing activities were observed in the identified Actinobacteria. Our results show that Actinobacteria from Parsık Cave might be good sources of industrial and biotechnological compounds. Furthermore, discover of new bioactive compounds from these bacteria is promising due to many unknown compounds observed in the GC-MS analysis and the high % of NRPS/PKS genes amplification.