Image processing method for automatic measurement of number of DNA breaks

Abstract

. The number of double - strand breaks can be evaluated from the change of average DNA length. The average DNA length is measured by the single - molecule observation method using fluorescence microscope. The measurement of DNA length in the microscope images is done manually by experienced operators and it is time consuming in many experi-ments. An image processing method using OpenCV library to measure length of DNA in fluorescence microscope images is developed in this paper. An automation of measurement using deep learning is also proposed.