Voltammetric bienzymatic sensor for sucrose determination in honey

IF 0.7 Q4 CHEMISTRY, ANALYTICAL Methods and Objects of Chemical Analysis Pub Date : 2021-01-01 DOI:10.17721/moca.2021.61-70
Anastasiia Kornii, Anna Borets, O. Tananaiko
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引用次数: 1

Abstract

An electrochemical sensor based on nanostructured planar carbon electrodes (nanoSPCE) modified with a SiO2 biocomposite film containing MnO2 particles and enzymes glucose oxidase and invertase was developed. MnO2 particles obtained by electrochemical deposition possessed electrocatalytic activity toward hydrogen peroxide. Glucose oxidase (GOx) and invertase (Inv) were encapsulated into SiO2 film by method of electroassisted deposition. The electrode modified with biocomposite film nanoSPCE/MnO2/GoX/Inv/SiO2 was electroactive toward sucrose. The indicator reaction was oxidation of H2O2 – the product of bienzymatic reaction which was catalyzed by MnO2 on the electrode surface. The linearity range of the calibration graph for the voltametric determination of sucrose using developed modified electrode is 0.017-0.342 mg/ml, the detection limit is 0.006 mg/ml. The obtained nanoSPCE/MnO2/GoX/Inv/SiO2 electrode demonstrated satisfactory stability of the analytical signal during one month of operation. The developed method was used for sucrose determination in the honey samples. The 50-fold molar excess of glucose and fructose does not interfere the determination of sucrose.
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测定蜂蜜中蔗糖的伏安双酶传感器
研制了一种基于纳米结构平面碳电极(nanoSPCE)的电化学传感器,该电极由含有二氧化锰颗粒和葡萄糖氧化酶和转化酶的SiO2生物复合膜修饰。电化学沉积得到的二氧化锰颗粒对过氧化氢具有电催化活性。采用电辅助沉积法将葡萄糖氧化酶(GOx)和转化酶(Inv)包封在SiO2薄膜中。纳米复合膜修饰的电极对蔗糖具有电活性。指示反应为MnO2在电极表面催化双酶反应产物H2O2的氧化。改进电极伏安法测定蔗糖的校准图线性范围为0.017 ~ 0.342 mg/ml,检出限为0.006 mg/ml。在一个月的运行过程中,所获得的纳米空间铈/二氧化锰/GoX/Inv/SiO2电极的分析信号具有良好的稳定性。该方法可用于蜂蜜中蔗糖含量的测定。50倍摩尔量的葡萄糖和果糖不会干扰蔗糖的测定。
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来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
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