Development of the Spectrophotometric Method for the Determination of Atorvastatin Calcium in Tablets by using Bromophenol Blue

IF 0.7 Q4 CHEMISTRY, ANALYTICAL Methods and Objects of Chemical Analysis Pub Date : 2022-01-01 DOI:10.17721/moca.2022.111-117
N. Shulyak, S. Protsyk, T. Kuche, L. Kryskiw, O. Poliak, L. Mosula, L. Logoyda
{"title":"Development of the Spectrophotometric Method for the Determination of Atorvastatin Calcium in Tablets by using Bromophenol Blue","authors":"N. Shulyak, S. Protsyk, T. Kuche, L. Kryskiw, O. Poliak, L. Mosula, L. Logoyda","doi":"10.17721/moca.2022.111-117","DOIUrl":null,"url":null,"abstract":"A simple, rapid, and inexpensive spectrophotometric method for the determination of atorvastatin calcium in tablets has been developed. It requires only one reagent (BPB) and suitable for routine pharmaceutical analysis with less sophisticated equipment. The procedure for atorvastatin determination is based on its interaction with bromophenol blue (BPB) in medium of organic solvent. As a result of the reaction, the intensity of the band of the doubly ionized form of the BPB with a maximum at 595 nm increases. The maximum analytical signal is observed in a solution of methanol, chloroform, or acetonitrile, while ethanol and ethyl acetate turned out to be unsuitable. The optimal concentration of BPB is 4.12·10-4 M. To study the stability, the absorbance of the obtained solution was measured under optimal conditions for 45 min. The stoichiometric ratio of components in the resulting associate, determined by the method of continuous variations, is 1:1. The calibration graph is linear in the range from 4.1 to 33 μmol/L. The calculated LOD and LOQ values were 1.36 μmol/L and 4.13 μmol/L, respectively. The systematic error of the method is statistically insignificant in the whole range of calibration graph. The developed method was successfully applied for the determination of atorvastatin in tablets.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Objects of Chemical Analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17721/moca.2022.111-117","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

A simple, rapid, and inexpensive spectrophotometric method for the determination of atorvastatin calcium in tablets has been developed. It requires only one reagent (BPB) and suitable for routine pharmaceutical analysis with less sophisticated equipment. The procedure for atorvastatin determination is based on its interaction with bromophenol blue (BPB) in medium of organic solvent. As a result of the reaction, the intensity of the band of the doubly ionized form of the BPB with a maximum at 595 nm increases. The maximum analytical signal is observed in a solution of methanol, chloroform, or acetonitrile, while ethanol and ethyl acetate turned out to be unsuitable. The optimal concentration of BPB is 4.12·10-4 M. To study the stability, the absorbance of the obtained solution was measured under optimal conditions for 45 min. The stoichiometric ratio of components in the resulting associate, determined by the method of continuous variations, is 1:1. The calibration graph is linear in the range from 4.1 to 33 μmol/L. The calculated LOD and LOQ values were 1.36 μmol/L and 4.13 μmol/L, respectively. The systematic error of the method is statistically insignificant in the whole range of calibration graph. The developed method was successfully applied for the determination of atorvastatin in tablets.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
溴酚蓝分光光度法测定片中阿托伐他汀钙含量的建立
建立了一种简单、快速、廉价的分光光度法测定阿托伐他汀片中钙含量的方法。它只需要一种试剂(BPB),适用于不太复杂的设备的常规药物分析。建立了在有机溶剂介质中测定阿托伐他汀与溴酚蓝(BPB)相互作用的方法。由于反应的结果,双电离形式的BPB的带强度增加,最大在595 nm处。在甲醇、氯仿或乙腈溶液中观察到最大的分析信号,而乙醇和乙酸乙酯则不适合。BPB的最佳浓度为4.12·10-4 m,为研究其稳定性,在最佳条件下测定所得溶液的吸光度45 min。通过连续变化法测定所得伴生物中各组分的化学计量比为1:1。在4.1 ~ 33 μmol/L范围内,标定曲线呈线性关系。定量限和定量限分别为1.36 μmol/L和4.13 μmol/L。该方法的系统误差在校正图的整个范围内均无统计学意义。该方法可用于阿托伐他汀片的含量测定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
期刊最新文献
Systematic Review and Guidelines for Management of Scrotal Inguinal Hernias. Study of the Biochemical Potential of Wild Fruit of the Caucasus Medar (Mespilus caucasics L.) in the Post-Harvest Period Chromatographic Determination of the Chemical Composition of Apple Chips Extract Development, Optimization, and Validation of a Novel HPLC Method for Simultaneous Quantification of Artesunate and Amodiaquine in Tablet Formulations Analytical Validation of a Reversed-Phase Ion Pairing HPLC-DAD Method for the Simultaneous Determination of Anthropogenic Pollutants
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1