Agrobacterium-Mediated Genetic Transformation System of Amorpha fruticosa using Callus from the Cotyledonary Node

Abstract

We used Agrobacterium-mediated infection of callus induced from the cotyledonary nodes of Amorpha fruticosa L. to study the β-glucuronidase gene (GUS)-integrated genetic transformation system. Transformed calluses were selected under 40 mg·L−1 kanamycin, differentiated into resistant adventitious buds, and developed into transformants. A single copy of gus was integrated in the genome of most T0 generation plants. Gus chemical staining analysis showed blue color in resistant calluses, adventitious buds, and the roots and leaves of transformed plants. This indicated gus overexpression driven by the 35S promotor and resultant β-glucuronidase activity. The genetic transformation system in this study could be used to study other functional genes of A. fruticosa and facilitate transgenic breeding for strain improvement. © 2022 Friends Science Publishers