ROTAVIRUS DISTRIBUTION IN OYSTERS CRASSOSTREA GIGAS TISSUE

J. Albarnaz, C. R. Kleemann, A. A. Corrêa, C. Figueiredo, C. Simões, E. Ferreira, C. Barardi
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Abstract

Shellfish are commonly associated with viral gastroenteritis outbreaks in human consumers. However, several attempts to depurate contaminated bivalve mollusks have been halted by increased persistence of viruses in mollusks’ tissues, mainly in digestive tract. RT-PCR and immunohistochemistry were applied to evaluate the localization of simian rotavirus SA11 strain in experimentally contaminated Crassostrea gigas oysters. Highest concentrations of SA11 persisted in digestive tract along 12-h experiment, whereas gills, mantle, labial palps and hemolymph presented fewer SA11. SA11 was found in close association with mucus filling oyster stomach. It can account for increased virus accumulation in digestive tract. DOI:  http://dx.doi.org/10.17525/vrr.v15i2.45
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轮状病毒在牡蛎、长牡蛎组织中的分布
贝类通常与人类消费者的病毒性肠胃炎暴发有关。然而,由于病毒在软体动物组织(主要是消化道)中持续存在的增加,一些净化受污染双壳类软体动物的尝试已经停止。采用RT-PCR和免疫组化技术对实验污染的长牡蛎中SA11类人猿轮状病毒的定位进行了评价。在实验的12 h内,SA11在消化道的浓度最高,而鳃、套、唇瓣和血淋巴的SA11浓度较低。SA11与牡蛎胃粘液充盈密切相关。它可以解释病毒在消化道中积聚的增加。DOI: http://dx.doi.org/10.17525/vrr.v15i2.45
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