Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

IF 0.8 4区 农林科学 Q3 AGRONOMY Ciencia Rural Pub Date : 2023-05-15 DOI:10.1590/0103-8478cr20220383
Mylla Christy da Silva Dufossé, Joelson Sousa Lima, Ana Paula Presley Oliveira Sampaio, Adrianne Maria Brito Pinheiro da Rosa, Josyane Brasil da Silva, C. M. Moraes, T. Roos
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Abstract

ABSTRACT: The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.
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空气性囊炎鸡致病性大肠杆菌毒力基因的分子鉴定及组织病理学分析
摘要:本研究鉴定了空气性囊炎鸡尸体中大肠杆菌引起的毒力基因和病理变化,并评估了组织病理学检查和聚合酶链反应(PCR)是否有效。从30只疑似空气囊炎的鸡身上采集了气管、肝脏和肺,这些鸡在检查线上被谴责。采用PCR同时鉴定两种毒力基因(iss和tsh基因)并进行组织病理学检测。PCR有效地表征了大肠杆菌分离株的基因典型特征,其中在三只鸟中同时发现了毒力基因iss和tsh。组织病理学检查发现气管(100%)、肺(90%)和肝脏(13.3%)以嗜中性粒细胞和单核细胞为主。肝脏是几乎没有被诊断出病变的器官。tsh和iss毒力基因的多重PCR结果表明,该方法在大肠杆菌分离株的鉴定中具有很大的潜力。未发生非特异性鉴定,因此有必要在鸟舍和家禽屠宰场使用鉴定和预防该剂的技术。
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来源期刊
Ciencia Rural
Ciencia Rural AGRONOMY-
CiteScore
1.70
自引率
0.00%
发文量
233
审稿时长
2-4 weeks
期刊介绍: The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.
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