{"title":"Second nitrate reductase of Dunaliella salina: functional redundancy or greater?","authors":"Z. Zalutskaya, Sofia Korkina, E. Ermilova","doi":"10.21685/1680-0826-2023-17-1-2","DOIUrl":null,"url":null,"abstract":"nitrate reductase (NR), which governs the reduction of nitrate to nitrite. The unicellular green alga Dunaliella salina is a special case, as its cells have two NRs. This makes D. salina attractive to study the mechanisms underlying the nitrate assimilation in photosynthetic unicellular organisms. Here we characterize the expression of the gene encoding the second NR, named as DsaNIA1 . Low levels of mRNA for DsaNIA1 are present in ammonia-grown cells. DsaNIA1 is a nitrate-and nitrite-inducible gene. Using spectrofluorometric assays with NO-sensitive fluorescence dye, we demonstrate nitrite-dependent NO synthesis by D. salina cells. Moreover, we found that the transcription of DsaNIA1 , but not DsaNIA2 , is under the inducing influence of NO-dependent pathway. Together, our data argue for the two differently regulated NR isoforms in D. salina .","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Protistology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21685/1680-0826-2023-17-1-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
nitrate reductase (NR), which governs the reduction of nitrate to nitrite. The unicellular green alga Dunaliella salina is a special case, as its cells have two NRs. This makes D. salina attractive to study the mechanisms underlying the nitrate assimilation in photosynthetic unicellular organisms. Here we characterize the expression of the gene encoding the second NR, named as DsaNIA1 . Low levels of mRNA for DsaNIA1 are present in ammonia-grown cells. DsaNIA1 is a nitrate-and nitrite-inducible gene. Using spectrofluorometric assays with NO-sensitive fluorescence dye, we demonstrate nitrite-dependent NO synthesis by D. salina cells. Moreover, we found that the transcription of DsaNIA1 , but not DsaNIA2 , is under the inducing influence of NO-dependent pathway. Together, our data argue for the two differently regulated NR isoforms in D. salina .
ProtistologyAgricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
1.50
自引率
0.00%
发文量
5
期刊介绍：
Protistology is one of the five "organism-oriented" journals for researchers of protistan material. The Journal publishes manuscripts on the whole spectrum of lower Eukaryote cells including protozoans, lower algae and lower fungi. Protistology publishes original papers (experimental and theoretical contributions), full-size reviews, short topical reviews (which are supposed to be somewhat "provocative" for setting up new hypotheses), rapid short communications, book reviews, symposia materials, historical materials, obituary notices on famous scientists, letters to the Editor, comments on and replies to published papers. Chronicles will present information about past and future scientific meetings, conferences, etc. THE PECULIARITIES OF THE JOURNAL - reviews, overviews and theoretical manuscripts on systematics, phylogeny, evolution and ecology of protists are favourably accepted - the manuscripts on multicellular organisms concerning their phylogenetic and taxonomic relationships with protists are also accepted - the size of manuscripts is usually not limited
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