Efecto comparativo del método de fractura mecánica y el método de corte en la viabilidad de las células de la pulpa dental humana de las 24 horas postexodoncia

Xiomara Flores-Cóndor, Esperanza R. Ayón-Haro
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引用次数: 1

Abstract

ABSTRACT Aim: In biomaterials and tissue engineering studies, dental pulp cells are used to regenerate or replace deficiencies of bone tissue in the oral cavity. To evaluate its clinical potential, cell culture models are employed, in which different extraction methods of the dental pulp are applied. Mainly, the tooth is broken by fracture mechanics and the cutting method to obtain the dental pulp. However, it is not clear yet which method is the most convenient. Therefore, the purpose of this study was to compare the effect of two methods of fracture mechanical and cutting methods on the dental pulp cell viability. Methodology: Dental pulp tissue was removed of fully developed and healthy teeth of 32 patients between 18 to 40 years, through two techniques: the methods of fracture mechanics (group 1) and cutting (group 2). To determine the cell viability, trypan blue dye was used. The cells were counted in the microscope, blue stained cells (dead) and birefringent (living). Results: Using the mechanical fracture method, 87.72 % of cell viability was obtained. While with the cutting method was achieved a lower viability 83.59 %. There was a significant difference (p = 0.006) when analyzing the data (n = 16 per group) under t-Student test. Conclusions: The use of mechanical fracture method for the extraction of the dental pulp tissue allows to obtain higher percentages of cell viability compared to cutting method.
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机械断裂法和切割法对人牙髓细胞拔牙24小时活力的比较影响
摘要目的:在生物材料和组织工程研究中,牙髓细胞被用来再生或替代口腔中缺乏的骨组织。为了评估其临床潜力,采用细胞培养模型,采用不同的牙髓提取方法。主要是通过断裂力学和切削法使牙齿断裂,得到牙髓。然而,目前尚不清楚哪种方法最方便。因此,本研究的目的是比较断裂机械和切割两种方法对牙髓细胞活力的影响。方法:采用断裂力学法(1组)和切割法(2组)两种方法,对32例年龄在18 ~ 40岁之间发育完整的健康牙齿进行牙髓组织切除。采用台锥蓝染色测定细胞活力。在显微镜下计数细胞,蓝色染色细胞(死亡)和双折射细胞(活)。结果:采用机械断裂法,细胞存活率达87.72%。而采用切削法获得的存活率较低,为83.59%。在t-Student检验下分析数据(n = 16 /组),差异有统计学意义(p = 0.006)。结论:与切割法相比,采用机械断裂法提取牙髓组织可获得更高的细胞存活率。
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来源期刊
CiteScore
0.20
自引率
0.00%
发文量
12
期刊介绍: La Revista Española de Cirugía Oral y Maxilofacial editada por Ergon es una publicación oficial de la Sociedad Española de Cirugía Oral y Maxilofacial (SECOM) Entre los objetivos de la revista se encuentran la difusión científica de la especialidad de Cirugía Oral y Maxilofacial y servir de medio de publicación para todos los miembros de la SECOM así como especialistas afines. Las áreas de interés de la revista son la científica, investigadora, informativa de eventos, becas, premios y revisiones bibliográficas.
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