Olívia Moreira, Bruna Berlande, Jéssica Cordeiro Queiroz de Souza, J. Candido, Lauren H. Jaeger, L. H. Adriano, Marco Antônio e Brito, Maria Patrícia do Nascimento, Patrícia Geraldo, P. Chellini, M. D. de Oliveira
{"title":"Capillary Electromigration Techniques Applied to Clinical Chemistry Investigations and Alternative Diagnostic Assays: Possibilities and Perspectives","authors":"Olívia Moreira, Bruna Berlande, Jéssica Cordeiro Queiroz de Souza, J. Candido, Lauren H. Jaeger, L. H. Adriano, Marco Antônio e Brito, Maria Patrícia do Nascimento, Patrícia Geraldo, P. Chellini, M. D. de Oliveira","doi":"10.21577/0103-5053.20230096","DOIUrl":null,"url":null,"abstract":"Capillary electromigration techniques can be understood as the ones used to separate neutral compounds, solvated ions and ionized species, considering the differentiated migration among them when an electrical field is applied within a capillary column filled with a background electrolyte. Several existing capillary electrophoresis setups provides the possibility of developing reliable quantitative assays of inorganic ions, organic acids, fatty acids, amino acids, carbohydrates, nucleic acids, proteins, hormones, vitamins, macromolecules, and more. Therefore, given the chemical complexity of bodily fluid matrices, capillary electrophoresis (CE) and its variations present themselves as an advantageous strategy for enhancing traditional clinical diagnostic methods and for the development of new ones. The recent advances in the study of the human metabolome associated with technological improvements towards medical applications make CE a very useful and versatile technique for clinical laboratory assays. Within this context, this review has the purpose of presenting some traditional methods used for exams of blood, urine, saliva, feces, and sweat, and how CE can be implemented as a real alternative to enable faster, automated, and cost-effective analysis with a comprehensive perspective that shall result in better diagnostic possibilities for patients, being a powerful tool for helping the physicians on the precision medicine achievements.","PeriodicalId":17257,"journal":{"name":"Journal of the Brazilian Chemical Society","volume":"1 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Brazilian Chemical Society","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.21577/0103-5053.20230096","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Capillary electromigration techniques can be understood as the ones used to separate neutral compounds, solvated ions and ionized species, considering the differentiated migration among them when an electrical field is applied within a capillary column filled with a background electrolyte. Several existing capillary electrophoresis setups provides the possibility of developing reliable quantitative assays of inorganic ions, organic acids, fatty acids, amino acids, carbohydrates, nucleic acids, proteins, hormones, vitamins, macromolecules, and more. Therefore, given the chemical complexity of bodily fluid matrices, capillary electrophoresis (CE) and its variations present themselves as an advantageous strategy for enhancing traditional clinical diagnostic methods and for the development of new ones. The recent advances in the study of the human metabolome associated with technological improvements towards medical applications make CE a very useful and versatile technique for clinical laboratory assays. Within this context, this review has the purpose of presenting some traditional methods used for exams of blood, urine, saliva, feces, and sweat, and how CE can be implemented as a real alternative to enable faster, automated, and cost-effective analysis with a comprehensive perspective that shall result in better diagnostic possibilities for patients, being a powerful tool for helping the physicians on the precision medicine achievements.
期刊介绍:
The Journal of the Brazilian Chemical Society embraces all aspects of chemistry except education, philosophy and history of chemistry. It is a medium for reporting selected original and significant contributions to new chemical knowledge.