Siti Fadilah Mohd Isa, Umi Marshida Abdul Hamid, Mohd Fakharul Zaman Raja Yahya
{"title":"Treatment with the combined antimicrobials triggers proteomic changes in P. aeruginosa-C. albicans polyspecies biofilms","authors":"Siti Fadilah Mohd Isa, Umi Marshida Abdul Hamid, Mohd Fakharul Zaman Raja Yahya","doi":"10.2306/scienceasia1513-1874.2022.020","DOIUrl":null,"url":null,"abstract":"Pseudomonas aeruginosa is known to coexist and interact with Candida albicans in the polyspecies biofilms. In our previous work, treatment with the combined antimicrobials has been shown to significantly inhibit this polyspecies biofilm; however, the proteomic changes associated with this biofilm inhibition remain not well understood. Thus, the present work was performed to determine the proteome profile of P. aeruginosa-C. albicans polyspecies biofilms following treatment with the combined antimicrobials. The P. aeruginosa-C. albicans polyspecies biofilms were developed in 6-well microplates at 37 °C for 24 h under aerobic and anaerobic conditions. Following the treatment with 40 mg/ml combined antimicrobials (20 mg/ml erythromycin:20 mg/ml nystatin), whole-cell proteomes from the polyspecies biofilms were analyzed by a combination of two-dimensional polyacrylamide gel electrophoresis (2D SDSPAGE) and mass spectrometry. On the other hand, the differentially expressed proteins were analyzed using STRING database. Results demonstrated that 3 and 9 P. aeruginosa proteins were differentially expressed in treated polyspecies biofilms under aerobic and anaerobic conditions, respectively. Based on the protein interaction network, several biological pathways such as carbohydrate metabolism, protein metabolism, and secondary metabolite metabolism in P. aeruginosa might be affected by the treatment with combined antimicrobials. There were no differentially expressed C. albicans proteins identified herein. It is possible that the inhibition of the polyspecies biofilms by the combined antimicrobial is associated with multiple biological pathways. The combined antimicrobials used in the present study may be useful to manage the diseases caused by P. aeruginosa-C. albicans polyspecies biofilms.","PeriodicalId":21577,"journal":{"name":"Scienceasia","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scienceasia","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.2306/scienceasia1513-1874.2022.020","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 3
Abstract
Pseudomonas aeruginosa is known to coexist and interact with Candida albicans in the polyspecies biofilms. In our previous work, treatment with the combined antimicrobials has been shown to significantly inhibit this polyspecies biofilm; however, the proteomic changes associated with this biofilm inhibition remain not well understood. Thus, the present work was performed to determine the proteome profile of P. aeruginosa-C. albicans polyspecies biofilms following treatment with the combined antimicrobials. The P. aeruginosa-C. albicans polyspecies biofilms were developed in 6-well microplates at 37 °C for 24 h under aerobic and anaerobic conditions. Following the treatment with 40 mg/ml combined antimicrobials (20 mg/ml erythromycin:20 mg/ml nystatin), whole-cell proteomes from the polyspecies biofilms were analyzed by a combination of two-dimensional polyacrylamide gel electrophoresis (2D SDSPAGE) and mass spectrometry. On the other hand, the differentially expressed proteins were analyzed using STRING database. Results demonstrated that 3 and 9 P. aeruginosa proteins were differentially expressed in treated polyspecies biofilms under aerobic and anaerobic conditions, respectively. Based on the protein interaction network, several biological pathways such as carbohydrate metabolism, protein metabolism, and secondary metabolite metabolism in P. aeruginosa might be affected by the treatment with combined antimicrobials. There were no differentially expressed C. albicans proteins identified herein. It is possible that the inhibition of the polyspecies biofilms by the combined antimicrobial is associated with multiple biological pathways. The combined antimicrobials used in the present study may be useful to manage the diseases caused by P. aeruginosa-C. albicans polyspecies biofilms.
期刊介绍:
ScienceAsia is a multidisciplinary journal publishing papers of high quality bimonthly, in printed and electronic versions, by the Science Society of Thailand under Royal Patronage and the National Research Council of Thailand. The journal publishes original research papers that provide novel findings and important contribution to broad area in science and mathematics. Areas covered include Biological Sciences and Biotechnology, Chemistry and Material Sciences, Environmental and Applied Sciences, and Mathematics and Physical Sciences. Manuscripts may report scientifically useful data, observations or model predictions, and/or provide a new scientific concept or a new explanation of published results. Submissions of materials of current scientific interest are highly welcome, provided that there is sufficient scientific merit. The journal will not accept manuscripts which have been published or are being considered for publication elsewhere, nor should manuscripts being considered by ScienceAsia be submitted to other journals. Submitted manuscripts must conform to the guidelines given in the Instructions for Authors