{"title":"EMBEDDING IN FILTER-STERILIZED ALGINATE ENHANCES BRASSICA OLERACEA L. PROTOPLAST CULTURE","authors":"A. Kiełkowska, A. Adamus","doi":"10.2478/ABCSB-2014-0018","DOIUrl":null,"url":null,"abstract":"The influence of sodium alginate sterilization on the viability and mitotic activity of embedded protoplasts was studied in protoplasts of Brassica oleracea subsp. alba and rubra isolated from hypocotyl tissue and leaves of seedlings or plants grown in vitro. Both leaf and hypocotyl-derived protoplasts were more viable and divided more frequently when embedded in filtrated alginate. Division frequency was highest in cv. Reball F1 and the mitotic activity of its protoplasts was three times higher when embedded in filtrated alginate (36.1 ± 6.8%) than when cultured in autoclaved alginate (10.9 ± 5.0%). Protoplast-derived calli colonies were transferred to solid regeneration media and plants of all tested accessions were obtained.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Biologica Cracoviensia Series Botanica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2478/ABCSB-2014-0018","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 5
Abstract
The influence of sodium alginate sterilization on the viability and mitotic activity of embedded protoplasts was studied in protoplasts of Brassica oleracea subsp. alba and rubra isolated from hypocotyl tissue and leaves of seedlings or plants grown in vitro. Both leaf and hypocotyl-derived protoplasts were more viable and divided more frequently when embedded in filtrated alginate. Division frequency was highest in cv. Reball F1 and the mitotic activity of its protoplasts was three times higher when embedded in filtrated alginate (36.1 ± 6.8%) than when cultured in autoclaved alginate (10.9 ± 5.0%). Protoplast-derived calli colonies were transferred to solid regeneration media and plants of all tested accessions were obtained.
期刊介绍：
ACTA BIOLOGICA CRACOVIENSIA Series Botanica is an English-language journal founded in 1958, devoted to plant anatomy and morphology, cytology, genetics, embryology, tissue culture, physiology, biochemistry, biosystematics, molecular phylogenetics and phylogeography, as well as phytochemistry. It is published twice a year.
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