HPLC ANALYSIS OF PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF AMORPHA FRUTICOSA L. EXTRACTS

Abstract

The present study focuses on the chemical and biological analysis of the ethanolic 70% v/v extracts obtained from Amorpha fruticosa leaves, branches and fruits. The total phenolic content determined by Folin-Ciocalteu method was the highest in leaves extract (159.5 mg/g). The HPLC-MS analysis indicated the presence of ferulic acid, luteolin and rutoside in all three extracts. The antioxidant activity was tested by the DPPH method and the results indicated a good scavenging activity (EC50 = 18.8 ± 0.2 μg/mL) for the fruit extract, followed by leaf extract (EC50 = 38.03 ± 0.75 μg/mL) and branch extract (EC50 = 221.16 ± 1.7 μg/mL). The antimicrobial activity evaluated by agar disc diffusion method indicated the inhibition of growth for Staphylococcus aureus and Sarcina lutea for all samples.