D. Pirone, Daniele G Sirico, L. Miccio, V. Bianco, M. Mugnano, D. D. Giudice, G. Pasquinelli, S. Valente, Silvia Lemma, L. Iommarini, I. Kurelac, P. Memmolo, P. Ferraro
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引用次数: 14
Abstract
The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets (LDs) in several regulatory mechanisms in living cells. LDs are dynamic organelles and therefore their complete characterization in terms of number, size, spatial positioning and relative distribution in the cell volume can shed light on the roles played by LDs. Until now, fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and specificity. However, such methods generally only provide 2D assays and partial measurements. Furthermore, both can be destructive and with low productivity, thus limiting analysis of large cell numbers in a sample. Here we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer, by using ovarian cancer cells and monocyte cell lines as models. A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is reported. The information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions. 3D D. Pirone, P. Memmolo, V. Bianco, L. Iom- marini and P. Ferraro analyzed and discussed the tomographic reconstruc-tions and data. I. Kurelac, S. Lemma and L. Iommarini performed FM ex- periments and data analysis; G. Pasquinelli and S. Valente performed the TEM; all the authors contributed to critical discussion of the results and contributed to write the manuscript. P. Ferraro supervised the research.
生物化学领域的最新发现强调了脂滴(ld)在活细胞的几种调节机制中的重要作用。ld是动态细胞器,因此它们在细胞体积中的数量、大小、空间定位和相对分布方面的完整表征可以揭示ld所起的作用。到目前为止,荧光显微镜和透射电子显微镜因其高灵敏度和特异性被评价为鉴定ld的金标准方法。然而,这种方法通常只提供二维分析和部分测量。此外,两者都具有破坏性且生产率低,因此限制了样品中大量细胞的分析。本文首次以卵巢癌细胞和单核细胞系为模型,利用层析相衬流式细胞仪展示了高通量的3D可视化和全LD表征能力。一种策略,检索显著参数的空间相关性和LD三维定位在每个细胞体积。通过这种新方法收集的信息可以让我们更深入地了解ld如何与细胞功能相关,并导致新的发现。3D D. Pirone, P. Memmolo, V. Bianco, L. Iom- marini和P. Ferraro对层析重建和数据进行了分析和讨论。I. Kurelac、S. Lemma和L. Iommarini进行调频实验和数据分析;G. Pasquinelli和S. Valente进行透射电镜观察;所有作者都参与了对结果的批判性讨论,并参与了论文的撰写。P. Ferraro监督了这项研究。
期刊介绍:
Opto-Electronic Advances (OEA) is a distinguished scientific journal that has made significant strides since its inception in March 2018. Here's a collated summary of its key features and accomplishments:
Impact Factor and Ranking: OEA boasts an impressive Impact Factor of 14.1, which positions it within the Q1 quartiles of the Optics category. This high ranking indicates that the journal is among the top 25% of its field in terms of citation impact.
Open Access and Peer Review: As an open access journal, OEA ensures that research findings are freely available to the global scientific community, promoting wider dissemination and collaboration. It upholds rigorous academic standards through a peer review process, ensuring the quality and integrity of the published research.
Database Indexing: OEA's content is indexed in several prestigious databases, including the Science Citation Index (SCI), Engineering Index (EI), Scopus, Chemical Abstracts (CA), and the Index to Chinese Periodical Articles (ICI). This broad indexing facilitates easy access to the journal's articles by researchers worldwide.
Scope and Purpose: OEA is committed to serving as a platform for the exchange of knowledge through the publication of high-quality empirical and theoretical research papers. It covers a wide range of topics within the broad area of optics, photonics, and optoelectronics, catering to researchers, academicians, professionals, practitioners, and students alike.