{"title":"Conserved Peptide with Therapeutic Potential to Overcome Nasopharyngeal Carcinoma","authors":"M. Rifa’i, S. Widyarti, N. Widodo","doi":"10.31436/IMJM.V13I1.490","DOIUrl":null,"url":null,"abstract":"ABSTRACT Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the upper lining epithelium of the nasopharynx. In this study, conserved peptide (Ulin-1) of Epstein-Barr virus constructed by Biomodelling and Biocomputation was tested for its ability to stimulate B cells to produce specific antibodies. Spleen cells were isolated and cultured with anti-CD3 and lipopolysaccharide (LPS), and treated or not treated with Ulin-1. Cell culture was harvested six days after incubation and analyzed by flow cytometry. Here, we demonstrated the ability of Ulin-1 to stimulate B cells to produce specific antibodies. The results of this study illustrate the importance of Ulin-1 engineered by Biomodelling and Biocomputation as both active and passive immunization agents against nasopharyngeal cancer. KEYWORDS: Nasopharyngeal carcinoma, Ulin-1, gp 350/220, in vitro.","PeriodicalId":53575,"journal":{"name":"International Medical Journal Malaysia","volume":"13 1","pages":""},"PeriodicalIF":0.3000,"publicationDate":"2020-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Medical Journal Malaysia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31436/IMJM.V13I1.490","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 2
Abstract
ABSTRACT Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the upper lining epithelium of the nasopharynx. In this study, conserved peptide (Ulin-1) of Epstein-Barr virus constructed by Biomodelling and Biocomputation was tested for its ability to stimulate B cells to produce specific antibodies. Spleen cells were isolated and cultured with anti-CD3 and lipopolysaccharide (LPS), and treated or not treated with Ulin-1. Cell culture was harvested six days after incubation and analyzed by flow cytometry. Here, we demonstrated the ability of Ulin-1 to stimulate B cells to produce specific antibodies. The results of this study illustrate the importance of Ulin-1 engineered by Biomodelling and Biocomputation as both active and passive immunization agents against nasopharyngeal cancer. KEYWORDS: Nasopharyngeal carcinoma, Ulin-1, gp 350/220, in vitro.
鼻咽癌(NPC)是一种鳞状细胞癌，发生在鼻咽部的上衬上皮。本研究通过生物建模和生物计算构建eb病毒的保守肽(Ulin-1)，测试其刺激B细胞产生特异性抗体的能力。分离脾细胞，用抗cd3和脂多糖(LPS)培养，用Ulin-1处理或不处理。培养6天后收获细胞，用流式细胞术分析。在这里，我们证明了Ulin-1刺激B细胞产生特异性抗体的能力。本研究结果说明了通过生物建模和生物计算工程设计的Ulin-1作为鼻咽癌主动和被动免疫剂的重要性。关键词:鼻咽癌，Ulin-1, gp 350/220，体外
期刊介绍：
International Medical Journal Malaysia (IMJM) is the official journal of the Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia. It serves primarily as a forum for education and intellectual discourse for health professionals namely in clinical medicine but covers diverse issues relating to medical ethics, professionalism as well as medical developments and research in basic medical sciences. It also serves the unique purpose of highlighting issues and research pertaining to the Muslim world. Contributions to the IMJM reflect its international and multidisciplinary readership and include current thinking across a range of specialties, ethnicities and societies.
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